Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/121235
Title: MECHANISTIC INSIGHTS INTO THE ACTION OF THE ACTIVATOR PROTEIN-L MEMBER C-JUN AT BOTH THE MOLECULAR AND PHYSIOLOGICAL LEVELS
Authors: XIE MIN
Keywords: c-Jun, phosphorylation, liver, fibrosis, stellate cell, hedgehog
Issue Date: 29-May-2015
Citation: XIE MIN (2015-05-29). MECHANISTIC INSIGHTS INTO THE ACTION OF THE ACTIVATOR PROTEIN-L MEMBER C-JUN AT BOTH THE MOLECULAR AND PHYSIOLOGICAL LEVELS. ScholarBank@NUS Repository.
Abstract: C-JUN, A MAJOR COMPONENT OF AP-1 TRANSCRIPTION FACTOR COMPLEX, IS INVOLVED IN A WIDE RANGE OF PHYSIOLOGICAL AND PATHOLOGICAL PROCESSES. HOWEVER THE GENE TARGETS THAT MEDIATE THESE PROCESSES REMAIN TO BE INVESTIGATED. ANALYSIS OF NOVEL C-JUN TARGETS BY WHOLE GENOME EXPRESSION PROFILES FROM EMBRYOS AND STRESSED PRIMARY MOUSE EMBRYONIC FIBROBLASTS (MEFS) SHOWED A SUBSTANTIAL NUMBER OF DIFFERENTIALLY EXPRESSED GENES BETWEEN C-JUN+/+ AND C-JUN-/- , WHILE ONLY A SMALL NUMBER OF GENES DIFFERED BETWEEN C-JUN+/+ AND C-JUNAA/AA. THUS C-JUN PHOSPHORYLATION IS REQUIRED ONLY FOR A SMALL SUBSET OF TARGET GENE TRANSCRIPTION. TO ELUCIDATE C-JUN¿S ROLE IN HEPATIC FIBROSIS (IDENTIFIED AS THE TOPMOST PATHWAY REGULATED BY C-JUN), WE UTILIZED C-JUN CONDITIONAL KNOCKOUT MICE TO INACTIVATE C-JUN IN (1) ADULT LIVER HEPATIC STELLATE CELLS (HSCS) AND (2) BOTH HEPATOCYTES AND HEMATOPOIETIC CELLS, BY USING COL-CREER AND MX-CRE TRANSGENIC MICE RESPECTIVELY. SURPRISINGLY, C-JUN ACTS AS A DUAL REGULATOR AS WE OB
URI: http://scholarbank.nus.edu.sg/handle/10635/121235
Appears in Collections:Ph.D Theses (Open)

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