Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/121102
Title: WATER QUALITY CONTROL USING DIVERSITY ORIENTED FLUORESCENCE LIBRARY APPROACH
Authors: XU WANG
Keywords: Fluorescence, Probe, Diversity-Oriented Synthesis, Water Quality, Disaggregation-induced Emission, Transition Metal Ion Detection
Issue Date: 5-May-2015
Citation: XU WANG (2015-05-05). WATER QUALITY CONTROL USING DIVERSITY ORIENTED FLUORESCENCE LIBRARY APPROACH. ScholarBank@NUS Repository.
Abstract: Fluorescence technique, especially small molecule fluorescent sensor, has vastly assisted our observation of this beautiful world and enhanced our understanding of the nature. Various fluorescent sensors have been designed for environmental contaminants and allowed their sensitive detection, such as heavy metal ion sensors, organic waste sensors and pathogen sensors, etc. However, due to the extreme diversity of these species, majority of the contaminants still lack potent sensors and the design can be difficult. To overcome this problem, diversity oriented fluorescence library approach (DOFLA) has been proposed and applied to address this issue. DOFLA has accumulated more than 10,000 fluorescent dyes and is the world?s largest toolbox for sensor development. In this thesis, we aim at applying DOFLA to monitor water quality, mainly various water contaminants. We started with construction and optimization of the high-throughput sensor development system of DOFLA toolbox. In this process, we have achieved phase I) an unbiased high-throughput screening platform of DOFLA and phase II) an image-based hyper-throughput screening box of DOFLA. Phase I platform has led us to discover the first turn-on fluorescent sensor for caffeine that works in aqueous solution, which can potentially be used to detect caffeine existence in various drinks and environment water. Phase II platform has allowed us to target various food safety and social security issues, including the first report of date rape drug sensors, bisphenol A sensor and milk fat sensor. Finally, with incorporation of the merits of both phase I and phase II platforms, we constructed a fluorescent sensor array that is capable of semi-quantifying multiple heavy metal ions. Incorporation of the sensor with upconversion nanoparticles has created a novel dye-assembled nanoparticle system selective for zinc ion and can be utilized in biological systems.
URI: http://scholarbank.nus.edu.sg/handle/10635/121102
Appears in Collections:Ph.D Theses (Open)

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