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|Title:||Nuclear morphometry and glutathione S-transferase π expression in breast cancer|
|Citation:||Huang, J.,Bay, B.-H.,Tan, P.-H. (2000-05). Nuclear morphometry and glutathione S-transferase π expression in breast cancer. Oncology Reports 7 (3) : 609-613. ScholarBank@NUS Repository.|
|Abstract:||Glutathione S-transferase π (GST-π) is a phase II detoxification enzyme whose expression is increased in estrogen receptor (ER)-poor breast cancers and in breast cancers resistant to certain chemotherapeutic agents. The aim of this study was to investigate the immunohistochemical expression of GST-π in invasive breast carcinoma and to correlate the findings with those of nuclear morphometry. Formalin-fixed paraffin-embedded tissue specimens obtained from 21 invasive breast cancers and 16 adjacent (benign) tissues were immunohistochemically stained using polyclonal anti-human GST-π antibody. There was positive (defined as >10% immunoreactive tumor cells) but variable expression of GST-π in 10 (48%) cases. Nuclear morphometry in these 10 tumors revealed immunoreactive malignant cells to be larger (mean area 41.7±1.0 μm2) and more rounded in form when compared with non-staining cancer cells (mean area 28.7±0.7 μm2). It was also observed that GST-π immunonegative tumor cells in GST-π expressing tumors had different morphologies from malignant cells in the remaining 11 (52%) cancers that were regarded as GST-π negative. Increased GST-π expression determined by the percentage of positively staining tumor cells, was found to be significantly correlated with increased variability in nuclear area and perimeter (Spearman's ρ=0.821, p=0.044 for both) in the subset of node-positive tumors. Our findings suggest that there exists two sub-populations of cancer cells with distinct nuclear morphologies in GST-ρ positive tumors; factors other than GST-ρ expression are likely to have a phenotypic effect on breast cancer cells; and there may be a special significance of this enzyme in axillary node-positive breast tumors.|
|Source Title:||Oncology Reports|
|Appears in Collections:||Staff Publications|
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