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|Title:||Induction of cytokine expression in the brain macrophages/amoeboid microglia of the fetal rat exposed to a teratogen|
|Keywords:||Brain macrophages (BM)/amoeboid microglia (AM)|
Interleukins (IL-1, IL-6)
Transforming growth factor-β (TGF-β)
Tumor necrosis factor-α (TNF-α)
|Citation:||Hao, A.-J.,Dheen, S.T.,Ling, E.-A. (2001-05-25). Induction of cytokine expression in the brain macrophages/amoeboid microglia of the fetal rat exposed to a teratogen. NeuroReport 12 (7) : 1391-1397. ScholarBank@NUS Repository.|
|Abstract:||Using in situ hybridization, RT-PCR, lectin histochemistry and immunohistochemistry, this study examined the time course expression and cellular localization of various cytokines including tumor necrosis factor-α (TNF-α), interleukins (IL-1, IL-6) and transforming growth factor-β (TGF-β) in fetal rat brain after a maternal injection of the teratogen cyclophosphamide (CP). Eight hours after CP injection, there was a marked increase in brain macrophages (BM)/amoeboid microglia (AM) in different areas of the fetal brain as determined by lectin histochemistry. Concomitant to this was the induction in mRNA level of TNF-α, which was progressively increased with time. TGF-α mRNA was undetectable until 24h had elapsed. Expression of IL-1 and IL-6 was undetectable at all stages. In situ hybridization combined with immunohistochemistry has shown the localization of TNF-α in BM/AM and neurons. Present results suggest that both TNF-α and TGF-β are involved in the progression of neural damage in the fetal brain induced by the teratogen. ©2001 Lippincott Williams & Wilkins.|
|Appears in Collections:||Staff Publications|
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