Please use this identifier to cite or link to this item:
Title: Induction of cytokine expression in the brain macrophages/amoeboid microglia of the fetal rat exposed to a teratogen
Authors: Hao, A.-J.
Dheen, S.T. 
Ling, E.-A. 
Keywords: Brain macrophages (BM)/amoeboid microglia (AM)
Fetal brain
Interleukins (IL-1, IL-6)
Transforming growth factor-β (TGF-β)
Tumor necrosis factor-α (TNF-α)
Issue Date: 25-May-2001
Citation: Hao, A.-J.,Dheen, S.T.,Ling, E.-A. (2001-05-25). Induction of cytokine expression in the brain macrophages/amoeboid microglia of the fetal rat exposed to a teratogen. NeuroReport 12 (7) : 1391-1397. ScholarBank@NUS Repository.
Abstract: Using in situ hybridization, RT-PCR, lectin histochemistry and immunohistochemistry, this study examined the time course expression and cellular localization of various cytokines including tumor necrosis factor-α (TNF-α), interleukins (IL-1, IL-6) and transforming growth factor-β (TGF-β) in fetal rat brain after a maternal injection of the teratogen cyclophosphamide (CP). Eight hours after CP injection, there was a marked increase in brain macrophages (BM)/amoeboid microglia (AM) in different areas of the fetal brain as determined by lectin histochemistry. Concomitant to this was the induction in mRNA level of TNF-α, which was progressively increased with time. TGF-α mRNA was undetectable until 24h had elapsed. Expression of IL-1 and IL-6 was undetectable at all stages. In situ hybridization combined with immunohistochemistry has shown the localization of TNF-α in BM/AM and neurons. Present results suggest that both TNF-α and TGF-β are involved in the progression of neural damage in the fetal brain induced by the teratogen. ©2001 Lippincott Williams & Wilkins.
Source Title: NeuroReport
ISSN: 09594965
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

checked on Oct 12, 2018

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.