Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/120726
DC Field | Value | |
---|---|---|
dc.title | Azide- and vanadate-sensitive M-phase alkalinity and cytosolic acidification of Chang liver cells | |
dc.contributor.author | Sit, K.-H. | |
dc.contributor.author | Paramanantham, R. | |
dc.contributor.author | Bay, B.-H. | |
dc.contributor.author | Wong, K.-P. | |
dc.date.accessioned | 2015-09-09T07:03:09Z | |
dc.date.available | 2015-09-09T07:03:09Z | |
dc.date.issued | 1994 | |
dc.identifier.citation | Sit, K.-H.,Paramanantham, R.,Bay, B.-H.,Wong, K.-P. (1994). Azide- and vanadate-sensitive M-phase alkalinity and cytosolic acidification of Chang liver cells. Japanese Journal of Pharmacology 65 (1) : 83-87. ScholarBank@NUS Repository. | |
dc.identifier.issn | 00215198 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/120726 | |
dc.description.abstract | Flow cytometric cell-by-cell evaluation of NH4Cl acidification of human Chang cells showed that at steady state, 3% of the cells remained alkalinized (> PH(i) 7) over an extended period (up to 80 min) despite the absence of extracellular Na+ and HCO3 -. In fluorescence microscopy, the acidification-resistant cells were characteristically rounded M-phase cells. Both mean cytosolic pH and M-phase alkalinity were however sensitive to (a) azide and oligomycin, inhibitors of F-ATPase (ATP synthase), and to (b) vanadium ions, the phosphate analogue of P-ATPase (ATP-hydrolyzing), in dose-dependent and time-dependent manners. Dead cell indices were constant at ≃ 10%. Thiocyanate chaotrophic anions, which cleave the V-ATPase structure, had no effect. Since ATP synthesizing F-ATPase (ATP synthase) is coupled to ATP-hydrolyzing P-ATPase as 'master-and-slave', azide- and oligomycin-sensitivity corroborated with vanadate-sensitivity in suggesting energized proton pumping modulating (a) M-phase alkalinity and (b) cytosolic pH, against acidification. | |
dc.source | Scopus | |
dc.subject | ATPase sensitivity (F-ATPase and P-ATPase) | |
dc.subject | Flow cytometric evaluation (BCECF ratio) | |
dc.subject | Proton pumping (M-phase and interphase) | |
dc.type | Article | |
dc.contributor.department | ANATOMY | |
dc.description.sourcetitle | Japanese Journal of Pharmacology | |
dc.description.volume | 65 | |
dc.description.issue | 1 | |
dc.description.page | 83-87 | |
dc.description.coden | JJPAA | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Staff Publications |
Show simple item record
Files in This Item:
There are no files associated with this item.
Google ScholarTM
Check
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.