Please use this identifier to cite or link to this item:
Title: On the role of endothelin-converting enzyme-1 (ECE-1) and neprilysin in human breast cancer
Authors: Smollich, M.
Götte, M.
Yip, G.W. 
Yong, E.-S.
Kersting, C.
Fischgräbe, J.
Radke, I.
Kiesel, L.
Wülfing, P.
Keywords: Breast cancer
Endothelin-converting enzyme
RO 67-7447
Issue Date: Dec-2007
Citation: Smollich, M., Götte, M., Yip, G.W., Yong, E.-S., Kersting, C., Fischgräbe, J., Radke, I., Kiesel, L., Wülfing, P. (2007-12). On the role of endothelin-converting enzyme-1 (ECE-1) and neprilysin in human breast cancer. Breast Cancer Research and Treatment 106 (3) : 361-369. ScholarBank@NUS Repository.
Abstract: Endothelin-1 (ET-1) and its receptors, ET AR and ET BR, are overexpressed in breast carcinomas. However, little is known about the relevance of endothelin-converting enzyme-1 (ECE-1) and ET-1 degrading neprilysin (NEP). In this study, expression of ECE-1 and NEP was determined in 600 breast cancer tissue samples by immunohistochemistry; staining results were correlated with clinicopathological parameters. For ECE-1 expression, we found a significant correlation with VEGF (P < 0.001) and ET AR expression (P = 0.048). While patients with ECE-1 overexpressing tumours had more frequent disease recurrence (P = 0.03), NEP overexpression correlated with improved disease-free survival (DFS) (P = 0.023) and less frequent metastasis (P = 0.046). Also, a decrease of NEP expression with malignant progression (G1-G3) was found. ECE-1 inhibition using the selective ECE-1 inhibitor RO 67-7447 in MCF-7 breast cancer cells led to a significantly decreased ET-1 expression and reduced cell invasiveness (54.3% of controls, P = 0.014). Our results indicate that overexpression of ECE-1 is associated with unfavourable outcome, whereas NEP positively influences survival. Thus, expression of ECE-1 and NEP may have prognostic relevance. Due to the anti-invasive effect of the selective ECE-1 inhibitor, targeting ECE-1 may represent an innovative option in future breast cancer therapy. © 2007 Springer Science+Business Media, LLC.
Source Title: Breast Cancer Research and Treatment
ISSN: 01676806
DOI: 10.1007/s10549-007-9516-9
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Jan 12, 2019


checked on Jan 2, 2019

Page view(s)

checked on Jan 18, 2019

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.