Please use this identifier to cite or link to this item: https://doi.org/10.1002/glia.20800
Title: Oct4 in expressed in human gliomas and promotes colony formation in glioma cells
Authors: Du, Z.
Jia, D.
Liu, S.
Wang, F.
Li, G.
Zhang, Y.
Cao, X.
Ling, E.-A. 
Hao, A.
Keywords: Glioma
Oct4
Proliferation
Self-renewal
Stat3
Issue Date: 2009
Source: Du, Z., Jia, D., Liu, S., Wang, F., Li, G., Zhang, Y., Cao, X., Ling, E.-A., Hao, A. (2009). Oct4 in expressed in human gliomas and promotes colony formation in glioma cells. GLIA 57 (7) : 724-733. ScholarBank@NUS Repository. https://doi.org/10.1002/glia.20800
Abstract: There is increasing evidence that self-renewal capacity of cancer cells is critical for carcinogenesis; hence, it is vital to examine the expression and involvement of self-renewal regulatory genes in these cells. Here, we reported that Oct4, a well-known regulator of self-renewal in embryonic stem cells, was highly expressed in human gliomas and glioma cell lines, and the expression levels were increased in parallel with increasing glioma grades. In in vitro cell cultures, Oct4 was only expressed in rat C6 glioma cells and rat neural stem cells but not in rat brain differentiated cells. Downregulation of Oct4 expression by RNA interference in C6 cells was associated with reduced cell proliferation and colony formation. Further analysis revealed that Oct4 could upregulate phosphorylation of Stat3 to promote tumor cell proliferation. Overexpression of Oct4 in C6 cells increased the expression of nestin but decreased the expression of GFAP suggesting that Oct4 might inhibit the differentiation of glioma cells. Our findings may provide further evidence for the stem cell theory of carcinogenesis. In contrast, the results might also imply that Oct4 contributes to the existence of undifferentiated cells in gliomas. © 2008 Wiley-Liss, Inc.
Source Title: GLIA
URI: http://scholarbank.nus.edu.sg/handle/10635/120670
ISSN: 08941491
DOI: 10.1002/glia.20800
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