Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/120557
Title: PHYSICAL RESPONSES OF CELLS TO GRATING TOPOGRAPHY VIA MECHANO-SENSING
Authors: WONG SUM THAI
Keywords: stem cells, focal adhesions, basal membrane bending, anisotropic rigidity, traction forces
Issue Date: 13-Aug-2014
Citation: WONG SUM THAI (2014-08-13). PHYSICAL RESPONSES OF CELLS TO GRATING TOPOGRAPHY VIA MECHANO-SENSING. ScholarBank@NUS Repository.
Abstract: IN TISSUE ENGINEERING, STEM CELLS PLAY AN IMPORTANT ROLE IN THE REGENERATION OF DISEASED TISSUES AND ORGANS. HUMAN MESENCHYMAL STEM CELLS (HMSCS) RESPOND TO BIOCHEMICAL AND PHYSICAL CUES TO DIFFERENTIATE INTO DIFFERENT LINEAGES. SURFACE TOPOGRAPHY INDUCES HMSC DIFFERENTIATION VIA CHANGES IN CELL SHAPE AND ADHESION. THE CURRENT WORK STUDIES PHYSICAL RESPONSES OF CELLS TO GRATING TOPOGRAPHY FROM A MECHANISTIC PERSPECTIVE. USING EXPERIMENTAL DATA AND MATHEMATICAL MODELING, THE PHYSICAL PARAMETERS INFLUENCING CELL-SUBSTRATE INTERACTION ARE IDENTIFIED AND USED TO EXPLAIN POSSIBLE MECHANISMS UNDERLYING MECHANO-SENSING. IT WAS FOUND THAT THE MECHANICAL AND GEOMETRICAL PROPERTIES OF GRATINGS ARE IMPORTANT IN REGULATING CELLULAR RESPONSES. WIDER GRATINGS WITH CURVE EDGES RESULTED IN THE BENDING OF THE BASAL MEMBRANE INTO GROOVES. THE ANISOTROPIC STRUCTURE OF THE GRATINGS ALSO ALTERED THE LOCAL RIGIDITY OF THE SUBSTRATE AND LED TO THE FORMATION OF LARGER FOCAL ADHESIONS AND STRONGER TRACTION FOR
URI: http://scholarbank.nus.edu.sg/handle/10635/120557
Appears in Collections:Ph.D Theses (Open)

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