Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/120141
Title: INVESTIGATION OF HYALURONAN AS AN ODOR DETECTED BY LARVAL ZEBRAFISH
Authors: KEE ZHI LING MICHELLE
Keywords: Olfactory system, hyaluronan, zebrafish
Issue Date: 12-Mar-2015
Source: KEE ZHI LING MICHELLE (2015-03-12). INVESTIGATION OF HYALURONAN AS AN ODOR DETECTED BY LARVAL ZEBRAFISH. ScholarBank@NUS Repository.
Abstract: Olfactory studies in zebrafish provided enormous insights into how olfaction occurs in an aquatic environment, triggering innate and stereotypical responses that allow survival in the vast habitat. One of the odors detected by zebrafish is an alarm substance from the lesioned skin of conspecifics, known as Schrecktoff. A feature of Schrecktoff is increased potency caused by heating, and glycan content. To further characterize Schreckstoff, through calcium imaging performed on transgenic zebrafish expressing the genetically encoded calcium indicator GCaMP, I demonstrated that HA evokes calcium responses in the olfactory system of zebrafish as young as 5 days post fertilization. This suggests HA can also function as an odor, in addition to its diverse size-dependent roles in cell signaling. I also describe and demonstrate the use of a microfluidic chip, as a ?proof-of-concept? to aid in characterizing olfactory sensory neurons responsible for detecting HA.
URI: http://scholarbank.nus.edu.sg/handle/10635/120141
Appears in Collections:Ph.D Theses (Open)

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