Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/120110
Title: ENGINEERED BONE-LIGAMENT INTERFACE FOR ANTERIOR CRUCIATE LIGAMENT REGENERATION: IN-VITRO STUDY
Authors: JIANG YUWEI
Keywords: silk fibroin scaffolds, bone marrow derived stem cell, gelatin microspheres, bone-ligament interface, growth factor delivery, in vitro
Issue Date: 13-Aug-2014
Source: JIANG YUWEI (2014-08-13). ENGINEERED BONE-LIGAMENT INTERFACE FOR ANTERIOR CRUCIATE LIGAMENT REGENERATION: IN-VITRO STUDY. ScholarBank@NUS Repository.
Abstract: Anterior cruciate ligament (ACL) injury is common in athletes. Tissue engineering method is used to regenerate the ACL, but it faces the problem of missing the bone-ligament interface, which is an important structure made up of bone, fibrocartilage and ligament. Fibrocartilage is further subdivided into calcified fibrocatilage and uncalcified fibrocatilage, contributing to the smooth transfer of mechanical force in ACL. In this thesis, the bone-ligament interface was engineered by making use of the multi-potency of bone marrow stem cell as well as the different bimolecular cues loaded composite silk fibroin scaffolds. In more details, the BMSC was pre-differentiated into tri-lineage(i.e, osteogenic, chondrogenic and fibroblastic) on individual scaffolds as well in individual induced medium for 1st three weeks, and then the tri-lineage pre-differentiated BMSC were integrated into one scaffold complex by suturing and co-cultured under the common medium for another three weeks, finally forming a bio-mimic transitional bone-ligament interface.
URI: http://scholarbank.nus.edu.sg/handle/10635/120110
Appears in Collections:Ph.D Theses (Open)

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