Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/119897
Title: CHARACTERIZATION OF THE LIPID PROFILE IN PLASMODIUM VIVAX
Authors: HUGO CARLOS SAMANO SANCHEZ
Keywords: Malaria, Bioinformatics, Lipidomics, Plasmodium vivax, phospholipids, Mass spectrometry
Issue Date: 24-Dec-2014
Citation: HUGO CARLOS SAMANO SANCHEZ (2014-12-24). CHARACTERIZATION OF THE LIPID PROFILE IN PLASMODIUM VIVAX. ScholarBank@NUS Repository.
Abstract: THE EMERGING LIPIDOMICS FIELD CAN BE USED TO UNDERSTAND KEY DIFFERENCES IN THE PHOSPHOLIPID COMPOSITION OF HUMAN PATHOGENS. WHILE RAPID ADVANCES IN THE TECHNOLOGY USED FOR HIGH-THROUGHPUT ANALYSES HAVE DECREASED THE COST AND TIME NEEDED FOR LIPIDOMICS DATA GENERATION, THEIR INTERPRETATION HAS BECOME A SIGNIFICANT BOTTLENECK. IN THIS THESIS WE PRESENT THE FIRST LIPIDOMICS ANALYSIS OF PLASMODIUM VIVAX INFECTED ERYTHROCYTES. IMPORTANTLY, WE PRESENTED AND DESCRIBED A PACKAGE-STRUCTURED CODE USED TO RAPIDLY PROCESS THE P. VIVAX LIPIDOMICS DATA. THIS PROGRAM ACQUIRES MONOISOTOPIC FEATURES FROM A MASS SPECTROMETRY EXPERIMENT TO BE PROCESSED IN A SUBSEQUENT TANDEM MS EXPERIMENT. IT ALSO IDENTIFIES PARTICULAR LIPID SPECIES AND GIVES HINTS ON LIPID CLASSIFICATION GIVEN A DATABASE OF PRECURSOR ION AND NEUTRAL LOSS SCANS. THIS NOVEL PIPELINE WAS USED TO IDENTIFY LIPIDS OVER EXPRESSED IN RETICULOCYTES INFECTED WITH P. VIVAX IN COMPARISON WITH NON-INFECTED RETICULOCYTES AND THE RESULTS ARE DISCUSSE
URI: http://scholarbank.nus.edu.sg/handle/10635/119897
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Thesis_HugoSamano.pdf2.89 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

91
checked on Oct 19, 2018

Download(s)

25
checked on Oct 19, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.