Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/119892
Title: ANALYSIS OF THE MECHANISMS OF HEPACAM-MEDIATED TUMOUR SUPPRESSION
Authors: WU MEIHUI
Keywords: hepaCAM, connexin 43, tumour suppression, gap junctions, fibronectin
Issue Date: 19-Jan-2015
Citation: WU MEIHUI (2015-01-19). ANALYSIS OF THE MECHANISMS OF HEPACAM-MEDIATED TUMOUR SUPPRESSION. ScholarBank@NUS Repository.
Abstract: hepaCAM (GlialCAM) is a novel immunoglobulin-like cell adhesion molecule that is frequently deleted in hepatocellular carcinoma and other solid cancers. hepaCAM is also expressed in the central nervous system and mutations in hepaCAM can give rise to the neurodegenerative disease megalencephalic leukoencephalopathy with subcortical cysts (MLC). In this study using the human glioblastoma cell line U373 MG, we show that hepaCAM interacts with the gap junction protein connexin 43, and promotes its trafficking to cellular junctions. hepaCAM expression in U373 MG cells also increases connexin 43 protein levels, not by increasing its transcription but by enhancing its stability. Mutations in hepaCAM which cause MLC, or neutralization of hepaCAM by an antagonistic antibody disrupt its interaction with connexin 43 at cellular junctions. We propose that hepaCAM-mediated trafficking of connexin 43 to cellular junctions increases cell-cell contact between glioblastoma cells, thus preventing them from escaping from the primary tumour and undergoing metastasis.
URI: http://scholarbank.nus.edu.sg/handle/10635/119892
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