Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/119620
Title: REPROGRAMMING OF HUMAN LUNG CANCER CELLS TO PLURIPOTENCY WITH DEFINED FACTORS AND THEIR CHARACTERIZATION
Authors: KONG CHIOU MEE
Keywords: Direct Reprogramming, Lung Cancer Cells, Pluripotency, Defined Factors, Cancer-derived iPSCs
Issue Date: 9-Jan-2015
Source: KONG CHIOU MEE (2015-01-09). REPROGRAMMING OF HUMAN LUNG CANCER CELLS TO PLURIPOTENCY WITH DEFINED FACTORS AND THEIR CHARACTERIZATION. ScholarBank@NUS Repository.
Abstract: REPORTS OF LOSS OF TUMORIGENICITY IN CANCER CELLS BY DIRECT REPROGRAMMING MAKING INDUCED PLURIPOTENT STEM CELLS (IPSCS) TECHNOLOGY AN EXCITING NEW AVENUE OF CANCER THERAPY. HOWEVER, DESPITE ITS POTENTIAL, A COMPENDIOUS STUDY OF THE EPIGENOME ALTERATIONS IN CANCER-DERIVED IPSCS HAS NOT BEEN REPORTED. USING HUMAN NON-SMALL CELL LUNG CANCER (NSCLC) CELLS, WE DEMONSTRATED THAT CANCER-DERIVED IPSCS RESEMBLE HUMAN EMBRYONIC STEM CELLS IN THEIR MORPHOLOGY, TRANSCRIPTIONAL PROFILES AND DIFFERENTIATION CAPABILITY. OUR WHOLE GENOME ANALYSES SHOWED THAT DIRECT REPROGRAMMING OF CANCER CELLS AMENDS THE ABERRANT EPIGENETIC SIGNATURES OF CANCER CELLS, THUS EXPLAINS THE LOSS OF TUMORIGENICITY AS WELL AS ATTENUATION OF DRUG SENSITIVITY IN CANCER CELLS UPON INDUCTION OF PLURIPOTENCY. ADDITIONALLY, OUR STUDY SHOWED THAT REPROGRAMMING OF CANCER CELLS ENRICHES A MINOR SUBPOPULATION WHICH IS MUTATION-FREE, AGAINST THE MAJORITY. OUR FINDINGS SUGGEST THAT REPROGRAMMING OF CANCER CELLS FOLLOWS A NON-STOCHASTIC
URI: http://scholarbank.nus.edu.sg/handle/10635/119620
Appears in Collections:Ph.D Theses (Open)

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