Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/119315
Title: EXPERIMENTS AND MODELING OF MOTILITY AND CHEMOTAXIS OF PSEUDOMONAS AERUGINOSA
Authors: CHEN QIAN
Keywords: Chemotaxis, motility, Pseudomonas aeruginosa, bacteria, model
Issue Date: 5-Aug-2014
Source: CHEN QIAN (2014-08-05). EXPERIMENTS AND MODELING OF MOTILITY AND CHEMOTAXIS OF PSEUDOMONAS AERUGINOSA. ScholarBank@NUS Repository.
Abstract: The ?run-and-tumble? model is well-established for the motility and chemotaxis of peritrichous bacteria such as Escherichia coli. However, for monotrichous bacteria constrained in a "run-and-reverse" motion, the mechanisms are still not well-understood. In this thesis, we combined experiments and computational modeling to study the motility and chemotaxis of monotrichous Pseudomonas aeruginosa. We developed an analytical platform called Bacterial Tethering Analysis Program (BTAP) that can accurately analyze tethering experiments of monotrichous bacteria such as P. aeruginosa. Using this, we first discovered a novel pause phase that allows P. aeruginosa to change directions. Next, we found that P. aeruginosa undergoes chemotaxis by rectifying the run-and-reverse motion, leading to longer runs in the direction of increasing chemoattractant. Finally, we proposed a theoretical model for how such rectification can be implemented in a molecular circuit involving the chemotaxis regulatory protein CheY and the flagella motor proteins. Simulations from the model show good agreement with experiments.
URI: http://scholarbank.nus.edu.sg/handle/10635/119315
Appears in Collections:Ph.D Theses (Open)

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