Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/119312
Title: INTERACTIONS OF NS5 THAT DRIVE ITS FUNCTION IN DENGUE PATHOGENESIS
Authors: TAY YUE FENG
Keywords: Dengue virus, NS5, antibody, NS3, protein-protein interaction, Flavivirus
Issue Date: 3-Feb-2015
Citation: TAY YUE FENG (2015-02-03). INTERACTIONS OF NS5 THAT DRIVE ITS FUNCTION IN DENGUE PATHOGENESIS. ScholarBank@NUS Repository.
Abstract: DENGUE VIRUS NONSTRUCTURAL PROTEIN 5 (NS5), CONTAINING BOTH METHYLTRANSFERASE AND RNA-DEPENDENT-RNA-POLYMERASE (RDRP) ACTIVITIES, IS RESPONSIBLE FOR PLUS- AND MINUS-STRAND VIRAL RNA SYNTHESIS AND CAN BE FOUND IN BOTH CYTOPLASM AND NUCLEUS OF INFECTED CELLS. CYTOPLASMIC NS5 INTERACTS WITH NS3 (PROTEASE/HELICASE) IN THE PERINUCLEAR REGION TO SYNTHESIZE THE VIRAL RNA GENOME WHEREAS NUCLEAR NS5 INTERACTS WITH IMPORTIN-A/?1 IN THE CYTOPLASM AND IS TRANSLOCATED TO THE NUCLEUS TO MEDIATE IN CELLULAR EVENTS THAT ARE NOT FULLY UNDERSTOOD. IN RECENT YEARS, NS3-NS5 AND NS5-IMPORTIN-A/?1 INTERACTION SITES HAVE BEEN PROPOSED TO BE ATTRACTIVE TARGETS FOR ANTIVIRAL DRUG DESIGN BUT MOLECULAR INTERACTION DETAILS ARE LACKING. THIS STUDY HAS FOUND THAT SUBCELLULAR LOCALIZATION OF NS5 VARIES IN A SEROTYPE DEPENDENT MANNER AND IDENTIFIED AMINO ACIDS NS3 570N, AND NS5 887KR AND 890RR TO BE IMPORTANT IN NS3-NS5 AND NS5-IMPORTIN-A/?1 INTERACTION, RESPECTIVELY. MUTATIONAL ANALYSIS WITH AN INFECTIOUS CDNA CLONE
URI: http://scholarbank.nus.edu.sg/handle/10635/119312
Appears in Collections:Ph.D Theses (Open)

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