Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/119248
Title: TOWARDS THE TREE-OF-LIFE OF SEPSIDAE(DIPTERA: CYCLORRHAPHA): EFFECTS OF TAXON AND GENE SAMPLING ON TOPOLOGICAL CONFLICT AND CLADE SUPPORT
Authors: LEI ZHAO
Keywords: Sepsidae, Phylogeny, Taxon sampling, Mitogenomics, Transcriptomics
Issue Date: 22-Aug-2014
Source: LEI ZHAO (2014-08-22). TOWARDS THE TREE-OF-LIFE OF SEPSIDAE(DIPTERA: CYCLORRHAPHA): EFFECTS OF TAXON AND GENE SAMPLING ON TOPOLOGICAL CONFLICT AND CLADE SUPPORT. ScholarBank@NUS Repository.
Abstract: Sepsidae (Diptera) is a clade of flies with ca. 320 described species. It is an important model for sexual selection research as well as an emerging system for the study of morphological innovations. For all comparative research, a species-rich and well-supported phylogenetic hypothesis is important. However, previous attempts at reconstructing the relationships revealed two major, interrelated problems: several weakly supported backbone nodes and topological conflict at these nodes when different tree optimality criteria were used. To address these problems, I first increased the taxon sample by 30% to 105 sepsid terminals. However, I find that the low support and topological conflict remained. I then increased gene sampling using Next-Generation Sequencing (NGS) techniques. The addition of mitochondrial genomes for 28 species did not resolve the support and conflict issues, but we find that transcriptomic data increased clade support and reduced topological conflict when two datasets with 603 and 1017 genes were analyzed. Finally, an analysis of the combined Sanger and NGS data yielded an overall well-supported and species-rich tree despite large amounts of missing data.
URI: http://scholarbank.nus.edu.sg/handle/10635/119248
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