Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/118911
Title: INVESTIGATIONS ON THE ROLE OF RAB23 ON CORTICOGENESIS IN THE MOUSE
Authors: HEIDI SHIOW CHYONG LIOU
Keywords: Rab23, radial migration, primary cilia formation
Issue Date: 14-Aug-2014
Citation: HEIDI SHIOW CHYONG LIOU (2014-08-14). INVESTIGATIONS ON THE ROLE OF RAB23 ON CORTICOGENESIS IN THE MOUSE. ScholarBank@NUS Repository.
Abstract: NEURONAL MIGRATION IN THE CEREBRAL CORTEX IS A FUNDAMENTAL PROCESS DURING DEVELOPMENT AND IF PERTURBED, CAN LEAD TO A VARIETY OF HUMAN DISEASES CALLED NEURONAL MIGRATION DISORDERS (NMD). HERE, WE REPORT FOR THE FIRST TIME THAT RAB23, A MEMBER OF THE RAB FAMILY OF SMALL GTPASES, AND MUTATED GENE PRODUCT OF THE OPEN BRAIN (OPB) MOUSE, IS REQUIRED FOR RADIAL MIGRATION OF CORTICAL PROJECTION NEURONS DURING CORTICAL DEVELOPMENT. KNOCK-DOWN OF RAB23 THROUGH RNA INTERFERENCE IN IN- UTERO ELECTROPORATED CORTICAL NEURONS OF THE DEVELOPING MOUSE EMBRYO DISTURBS THEIR RADIAL MIGRATION. FURTHERMORE, WE SHOW THAT RAB23-DEFICIENCY IN DEVELOPING PRIMARY CORTICAL NEURONS DISTURBS THE FORMATION OF PRIMARY CILIA, A KEY CELLULAR SENSOR OF EXTERNAL CUES, IN VITRO, AND IN DEVELOPING CORTICAL PROJECTION NEURONS IN VIVO. BOTH PHENOTYPES, RADIAL MIGRATION AND CILIOGENESIS IN VIVO, CAN BE RESCUED BY RAB23 CO- EXPRESSION. IN A PILOT STUDY, AN ASSOCIATION OF RAB23-SILENCING IN CORTICAL PROJECTION NEURONS DURING
URI: http://scholarbank.nus.edu.sg/handle/10635/118911
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