Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/118492
Title: ONCOGENICITY OF ZEBRAFISH MYC GENES IN HEPATOCARCINOGENSIS BY INDUCIBLE TRANSGENIC EXPRESSION
Authors: SUN LILI
Keywords: zebrafish, hepatocellular carcinoma, myca, mycb, tp53
Issue Date: 27-Jun-2014
Citation: SUN LILI (2014-06-27). ONCOGENICITY OF ZEBRAFISH MYC GENES IN HEPATOCARCINOGENSIS BY INDUCIBLE TRANSGENIC EXPRESSION. ScholarBank@NUS Repository.
Abstract: HEPATOCELLULAR CARCINOMA (HCC) IS ONE OF THE TOP LETHAL CANCERS NOWADAYS. DEREGULATION OF MYC IN HCC IS FREQUENTLY DETECTED AND ALWAYS CORRELATED WITH POOR PROGNOSIS. IN THIS STUDY, WE FOUND THAT BOTH OF THE TWO ZEBRAFISH MYC ORTHOLOGS, MYCA AND MYCB, ARE ONCOGENIC. MOREOVER, THE TRANSGENIC EXPRESSION OF MYCA IN HEPATOCYTES CAUSED FAST ONSET OF TUMORS WITH DIVERGENT PHENOTYPES AND 5% FISH DEVELOPED MULTINODULAR HCC WITH CIRRHOSIS AFTER 8 MONTHS EXPRESSION OF MYCA. APPARENT APOPTOSIS WAS ALSO OBSERVED WITH MYCA EXPRESSION. BUT INTRODUCING HOMOZYGOUS TP53M214K MUTATION INTO THE MYCA TRANSGENIC FISH COULD INHIBIT APOPTOSIS AND ACCELERATE TUMOR PROGRESSION. NONETHELESS, THE MALIGNANT STATUS OF HEPATOCYTES RELIED ON CONSISTENT EXPRESSION OF MYCA, WHICH FAST REGRESSED AFTER WITHDRAW OF MYCA EXPRESSION, EVEN IN FISH WITH TP53M214K MUTATION. TAKE TOGETHER, OUR DATA DEMONSTRATE THE ROBUST ONCOGENICITY OF ZEBRAFISH MYCA, AND ALSO SUGGEST ZEBRAFISH LIVER CANCER MODEL A POTENTIAL APPLICATION IN HU
URI: http://scholarbank.nus.edu.sg/handle/10635/118492
Appears in Collections:Ph.D Theses (Open)

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