Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/118131
Title: PRIMATE/HUMAN-SPECIFIC FEATURES AND FUNCTION OF TROPHOBLAST LINEAGE
Authors: MEHRAN RAHMANI
Keywords: Embryo implantation, syncytiotrophoblast, Placenta evolution, Primate-specific, Genomic technologies, Human Embryonic Stem Cell
Issue Date: 24-Jan-2014
Citation: MEHRAN RAHMANI (2014-01-24). PRIMATE/HUMAN-SPECIFIC FEATURES AND FUNCTION OF TROPHOBLAST LINEAGE. ScholarBank@NUS Repository.
Abstract: UNDERSTANDING THE UNDERLYING MOLECULAR EVENTS IN THE PRIMATE EVOLUTION HAS BEEN A MAJOR CHALLENGE FOR ADDRESSING THE CAUSE OF HUMAN EMBRYO IMPLANTATION DISORDERS AND INFERTILITY PROBLEMS. TO ADDRESS THIS, AN IN VITRO SYSTEM HAS BEEN DEVELOPED WITH THE USE OF HUMAN EMBRYONIC STEM CELLS DIFFERENTIATED TOWARDS THE SYNCYTIOTROPHOBLAST LINEAGE. I ARGUE THIS MODEL RECAPITULATES THE FORMATION OF THE PRIMITIVE SYNCYTIUM. BY APPLYING HIGH THROUGHPUT SEQUENCING METHODS, WE FOUND WITHIN THE HUMAN SYNCYTIOTROPHOBLAST PROTEIN CODING TRANSCRIPTOME; PRIMATE-SPECIFIC FEATURES RESULTED FROM RECENT GENE DUPLICATIONS, POSITIVE SELECTION AND CO-OPTION OF ENDOGENOUS RETROVIRAL GENES. EXTENSIVE NOVEL REGULATORY CHANGE ALTERING GENE EXPRESSION WAS IDENTIFIED. THESE CIS-REGULATORY NOVELTIES AROSE BOTH THROUGH APPARENT ACCUMULATION OF SINGLE NUCLEOTIDE MUTATION AND THE CO-OPTION OF ERV LONG TERMINAL REPEATS (LTRS). THESE RESULTS PROVIDE A MECHANISTIC INSIGHT IN THE EVOLUTIONARY EVENTS, WHICH IS INVOLVED IN THE
URI: http://scholarbank.nus.edu.sg/handle/10635/118131
Appears in Collections:Ph.D Theses (Open)

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