Please use this identifier to cite or link to this item:
Title: Proteomic approach identifies HSP27 as an interacting partner of the hepatitis C virus NS5A protein
Authors: Choi, Y.-W.
Tan, Y.-J.
Lim, S.G. 
Hong, W. 
Goh, P.-Y.
Keywords: HCV
Issue Date: 2004
Citation: Choi, Y.-W., Tan, Y.-J., Lim, S.G., Hong, W., Goh, P.-Y. (2004). Proteomic approach identifies HSP27 as an interacting partner of the hepatitis C virus NS5A protein. Biochemical and Biophysical Research Communications 318 (2) : 514-519. ScholarBank@NUS Repository.
Abstract: Chronic infection by HCV is closely correlated with liver diseases such as cirrhosis, steatosis, and hepatocellular carcinoma. To understand how long-term interaction between HCV and the host leads to pathogenesis, we identified cellular proteins that interact with NS5A and NS5B using a biochemical approach. Stable cell lines that express flag-NS5A or flag-NS5B under tetracycline induction were generated. The induced flag-tagged proteins were immunoprecipitated (IP'd) and associated proteins separated on 2D gels. Protein spots that specifically co-IP'd with NS5A or NS5B were identified by mass spectrometry. HSP27 was identified as a protein that specifically co-IP'd with NS5A but not with NS5B. The N-terminal regions of NS5A (a.a. 1-181) and HSP27 (a.a. 1-122) were defined to be the domains that interact with each other. HSP27 is generally distributed in the cytoplasm. When heat shocked, HSP27 is concentrated in the ER where NS5A is co-localized. © 2004 Elsevier Inc. All rights reserved.
Source Title: Biochemical and Biophysical Research Communications
ISSN: 0006291X
DOI: 10.1016/j.bbrc.2004.04.052
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Dec 11, 2018

Page view(s)

checked on Oct 19, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.