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Title: CD81 engineered with endocytotic signals mediates HCV cell entry: Implications for receptor usage by HCV in vivo
Authors: Tan, Y.-J.
Lim, S.-P.
Goh, P.-Y.
Lim, S.G. 
Tan, Y.H.
Hong, W. 
Ng, P.
Keywords: CD81
Chimeric receptors
Hepatitis C virus (HCV)
Low-density lipoprotein receptor
Transferrin receptor
Virus replication
Issue Date: 2003
Source: Tan, Y.-J., Lim, S.-P., Goh, P.-Y., Lim, S.G., Tan, Y.H., Hong, W., Ng, P. (2003). CD81 engineered with endocytotic signals mediates HCV cell entry: Implications for receptor usage by HCV in vivo. Virology 308 (2) : 250-269. ScholarBank@NUS Repository.
Abstract: Although CD81 has been shown to bind HCV E2 protein, its role as a receptor for HCV remains controversial. In this study, we constructed two CD81 chimeras by linking the cytoplasmic domains of recycling surface receptors, low-density lipoprotein receptor (LDLR), and transferrin receptor (TfR), respectively, to CD81 and compared their internalization properties to wild-type CD81. Binding experiments with anti-hCD81 antibody showed that cell-surface CD81 chimeric receptors were internalized much more efficiently than wild-type CD81. In addition, CD81 chimeras, but not wild-type CD81, could internalize recombinant E2 protein and E2-enveloped viral particles from the serum of HCV-infected patients into Huh7 liver cells. The latter resulted in persistent positive-strand viral RNA and accumulation of replication intermediates, negative-strand viral RNA, in the infected cells, suggesting that the internalized viruses have undergone replication. Therefore, it appeared that CD81, possibly in association with a liver-specific endocytotic protein(s), represents one of the pathways by which HCV can infect hepatocytes. © 2003 Elsevier Science (USA). All rights reserved.
Source Title: Virology
ISSN: 00426822
DOI: 10.1016/S0042-6822(02)00136-8
Appears in Collections:Staff Publications

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