Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/117493
Title: INVESTIGATING THE ROLES OF ZFP322A AND PATZ1 IN ES CELL IDENTITY AND REPROGRAMMING
Authors: MA HUI
Keywords: ESC, Zinc finger protein, Zfp322a, Patz1, reprogramming, pluripotency
Issue Date: 13-Aug-2014
Source: MA HUI (2014-08-13). INVESTIGATING THE ROLES OF ZFP322A AND PATZ1 IN ES CELL IDENTITY AND REPROGRAMMING. ScholarBank@NUS Repository.
Abstract: Embryonic stem cells (ESCs) derived from the inner cell mass (ICM) of murine blastocysts are characterised by their ability to self-renew and their potential to differentiate into many different cell types. These unique properties are governed by a complex transcription regulatory network, including master regulators Oct4/Sox2/Nanog and other pluripotency factors. Interestingly, depletion of a single transcription factor of this network will alter ESC pluripotency. Thus it would be vital to decipher the delicate transcription regulation of pluripotent state in mESCs. The importance of this transcription regulatory network was highlighted by the remarkable discovery that overexpression of transcription factors can reprogram the differentiated somatic cells to pluripotent cells, i.e. induced pluripotent stem cells (iPSCs). This process was therefore known as somatic cell reprogramming. Recent studies have found numerous transcription factors important for ESC identity maintainance or pluripotency induction in somatic cell reprogramming, including many zinc finger proteins. In this thesis, we identified two zinc finger proteins, Zfp322a and Patz1, which are important regulators of ES cell identity Our results revealed Zfp322a as a novel pluripotency factor which is not only required for maintaining ES cell identity but also can enhance somatic cell reprogramming. On the other hand, Patz1, though previously reported as a pluripotency factor in mESCs, modulates the reprogramming process in a dosage-dependent manner, possibly through its regulation of c-Myc, cell senescence, mesenchymal to epithelial transition (MET) and chromatin modification. Together, these results highlight the novel functions of zinc finger proteins in ES cell identity and reprogramming.
URI: http://scholarbank.nus.edu.sg/handle/10635/117493
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