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Title: Structural basis for the interaction of unstructured neuron specific substrates neuromodulin and neurogranin with calmodulin
Authors: Kumar, V.
Chichili, V.P.R.
Zhong, L.
Tang, X. 
Velazquez-Campoy, A.
Sheu, F.-S. 
Seetharaman, J.
Gerges, N.Z.
Sivaraman, J. 
Issue Date: 2013
Source: Kumar, V., Chichili, V.P.R., Zhong, L., Tang, X., Velazquez-Campoy, A., Sheu, F.-S., Seetharaman, J., Gerges, N.Z., Sivaraman, J. (2013). Structural basis for the interaction of unstructured neuron specific substrates neuromodulin and neurogranin with calmodulin. Scientific Reports 3 : -. ScholarBank@NUS Repository.
Abstract: Neuromodulin (Nm) and neurogranin (Ng) are neuron-specific substrates of protein kinase C (PKC). Their interactions with Calmodulin (CaM) are crucial for learning and memory formation in neurons. Here, we report the structure of IQ peptides (24aa) of Nm/Ng complexed with CaM and their functional studies with full-length proteins. Nm/Ng and their respective IQ peptides are intrinsically unstructured; however, upon binding with CaM, IQ motifs adopt a helical conformation. Ser41 (Ser36) of Nm (Ng) is located in a negatively charged pocket in the apo CaM and, when phosphorylated, it will repel Nm/Ng from CaM. These observations explain the mechanism by which PKC-induced Ser phosphorylation blocks the association of Nm/Ng with CaM and interrupts several learning-and memory-associated functions. Moreover, the present study identified Arg as a key CaM interacting residue from Nm/Ng. This residue is crucial for CaM-mediated function, as evidenced by the inability of the Ng mutant (Arg-to-Ala) to potentiate synaptic transmission in CA1 hippocampal neurons.
Source Title: Scientific Reports
ISSN: 20452322
DOI: 10.1038/srep01392
Appears in Collections:Staff Publications

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