Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.stem.2013.09.003
Title: Dynamic analysis of gene expression and genome-wide transcription factor binding during lineage specification of multipotent progenitors
Authors: May, G.
Soneji, S.
Tipping, A.J.
Teles, J.
McGowan, S.J.
Wu, M. 
Guo, Y.
Fugazza, C.
Brown, J.
Karlsson, G.
Pina, C.
Olariu, V.
Taylor, S.
Tenen, D.G.
Peterson, C.
Enver, T.
Issue Date: 5-Dec-2013
Citation: May, G., Soneji, S., Tipping, A.J., Teles, J., McGowan, S.J., Wu, M., Guo, Y., Fugazza, C., Brown, J., Karlsson, G., Pina, C., Olariu, V., Taylor, S., Tenen, D.G., Peterson, C., Enver, T. (2013-12-05). Dynamic analysis of gene expression and genome-wide transcription factor binding during lineage specification of multipotent progenitors. Cell Stem Cell 13 (6) : 754-768. ScholarBank@NUS Repository. https://doi.org/10.1016/j.stem.2013.09.003
Abstract: We used the paradigmatic GATA-PU.1 axis to explore, at the systems level, dynamic relationships between transcription factor (TF) binding and global gene expression programs as multipotent cells differentiate. We combined global ChIP-seq of GATA1, GATA2, and PU.1 with expression profiling during differentiation to erythroid and neutrophil lineages. Our analysis reveals (1) differential complexity of sequence motifs bound by GATA1, GATA2, and PU.1; (2) the scope and interplay of GATA1 and GATA2 programs within, and during transitions between, different cell compartments, and the extent of their hard-wiring by DNA motifs; (3) the potential to predict gene expression trajectories based on global associations between TF-binding data and target gene expression; and (4) how dynamic modeling of DNA-binding and gene expression data can be used to infer regulatory logic of TF circuitry. This rubric exemplifies the utility of this cross-platform resource for deconvoluting the complexity of transcriptional programs controlling stem/progenitor cell fate in hematopoiesis. © 2013 Elsevier Inc.
Source Title: Cell Stem Cell
URI: http://scholarbank.nus.edu.sg/handle/10635/116987
ISSN: 19345909
DOI: 10.1016/j.stem.2013.09.003
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

35
checked on Jun 14, 2018

WEB OF SCIENCETM
Citations

32
checked on May 7, 2018

Page view(s)

44
checked on Jun 1, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.