Please use this identifier to cite or link to this item:
https://doi.org/10.1371/journal.pone.0050786
DC Field | Value | |
---|---|---|
dc.title | Combination Therapy with Gossypol Reveals Synergism against Gemcitabine Resistance in Cancer Cells with High BCL-2 Expression | |
dc.contributor.author | Wong, F.Y. | |
dc.contributor.author | Liem, N. | |
dc.contributor.author | Xie, C. | |
dc.contributor.author | Yan, F.L. | |
dc.contributor.author | Wong, W.C. | |
dc.contributor.author | Wang, L. | |
dc.contributor.author | Yong, W.-P. | |
dc.date.accessioned | 2014-12-12T07:59:38Z | |
dc.date.available | 2014-12-12T07:59:38Z | |
dc.date.issued | 2012-12-04 | |
dc.identifier.citation | Wong, F.Y., Liem, N., Xie, C., Yan, F.L., Wong, W.C., Wang, L., Yong, W.-P. (2012-12-04). Combination Therapy with Gossypol Reveals Synergism against Gemcitabine Resistance in Cancer Cells with High BCL-2 Expression. PLoS ONE 7 (12) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0050786 | |
dc.identifier.issn | 19326203 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/116954 | |
dc.description.abstract | Although gemcitabine is highly active in several cancer types, intrinsic and acquired drug resistance remains a major challenge. Overexpression of Bcl-2 has been associated with gemcitabine resistance. The aim of this study is to determine whether gossypol can overcome gemcitabine resistance in cell lines with high level of Bcl-2 expression in combination drug therapy. Our study demonstrated that in 10 cell lines derived from different cancers, high Bcl-2 baseline expression was observed in cell lines that were resistant to gemcitabine (GEM-R). Furthermore, synergistic effect of combination therapy was observed in gemcitabine-resistant (GEM-R) cell lines with high Bcl-2 expression, but not in a gemcitabine-sensitive (GEM-S) cell lines regardless of Bcl-2 expression. Gossypol treatment resulted in the decrease of anti-apoptotic genes such as Bcl-2 and Bcl-xl and an upregulation of the pro-apoptotic gene, Noxa. Furthermore, the addition of gossypol to gemcitabine resulted in lower expressions of anti-apoptotic genes compared to gemcitabine alone. Gene expression profiling in GEM-R and GEM-S cell lines suggest that anti-apoptotic genes such as pAkt and PI3KR2 may play important role in gemcitabine resistance, while pro-apoptotic Bcl-2 related genes (Bad, Caspase-6 and Calpain-1) may regulate synergistic interaction in combination therapy. © 2012 Wong et al. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1371/journal.pone.0050786 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | CANCER SCIENCE INSTITUTE OF SINGAPORE | |
dc.description.doi | 10.1371/journal.pone.0050786 | |
dc.description.sourcetitle | PLoS ONE | |
dc.description.volume | 7 | |
dc.description.issue | 12 | |
dc.description.page | - | |
dc.identifier.isiut | 000312108500031 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications |
Show simple item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_1371_journal_pone_0050786.pdf | 1.47 MB | Adobe PDF | OPEN | Published | View/Download |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.