Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jchromb.2010.07.015
Title: A sensitive and specific liquid chromatography-tandem mass spectrometric method for determination of belinostat in plasma from liver cancer patients
Authors: Wang, L.-Z. 
Chan, D.
Yeo, W.
Wan, S.-C.
Chan, S.
Chan, A.
Lee, S.-C.
Lee, H.-S.
Goh, B.-C.
Keywords: Belinostat
Belinostat glucuronide
Human plasma
LC-MS/MS
Issue Date: Sep-2010
Citation: Wang, L.-Z., Chan, D., Yeo, W., Wan, S.-C., Chan, S., Chan, A., Lee, S.-C., Lee, H.-S., Goh, B.-C. (2010-09). A sensitive and specific liquid chromatography-tandem mass spectrometric method for determination of belinostat in plasma from liver cancer patients. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 878 (26) : 2409-2414. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jchromb.2010.07.015
Abstract: A novel, sensitive and reliable liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the determination of belinostat (PXD101) in human plasma. Oxamflatin was used as the internal standard. Liquid-liquid extraction of the plasma sample was performed using tert-butyl methyl ether as the organic solvent. Chromatographic separation was achieved on a BDS Hypersil C18 column (2.1. mm × 100. mm, 5μm) using gradient elution mode using 0.05% formic acid in water and 0.05% formic acid in acetonitrile as solvents A and B, respectively, 60/40. The run time was 6. min. The mass spectrometer was operated under a positive electrospray ionization condition and a multiple reaction monitoring mode. An excellent linear calibration was achieved in the range of 0.5-1000. ng/mL. An average recovery of belinostat for four quality controls was 72.6% and the recovery of the internal standard at 1000. ng/mL was 67.8%. The intra-day and inter-day precisions for belinostat were ≤8.0 and ≤10.3%, respectively, and their accuracy ranged from 100.2 to 106.7%. No significant matrix effect was identified. In analysis of patient samples, belinostat glucuronide was identified and baseline separated from belinostat. This well-validated assay has been applied for quantification of belinostat in plasma samples within 24. h after the start of infusion for Asian hepatocellular carcinoma patients in a dose escalation study. © 2010 Elsevier B.V.
Source Title: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
URI: http://scholarbank.nus.edu.sg/handle/10635/116911
ISSN: 15700232
DOI: 10.1016/j.jchromb.2010.07.015
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