Please use this identifier to cite or link to this item:
Title: Promoter region of the transcriptional unit for human α1-chimaerin, a neuron-specific GTPase-activating protein for p21rac
Authors: Dong, J.-M. 
Smith, P.
Hall, C.
Lim, L.
Keywords: CAT vectors
CCAAT-binding protein
neuronal gene expression
promoter analysis
Issue Date: 1995
Citation: Dong, J.-M., Smith, P., Hall, C., Lim, L. (1995). Promoter region of the transcriptional unit for human α1-chimaerin, a neuron-specific GTPase-activating protein for p21rac. European Journal of Biochemistry 227 (3) : 636-646. ScholarBank@NUS Repository.
Abstract: α1-chimaerin is a neuron-specific GTPase-activating protein for p21rac, a protein involved in morphological events. The mRNA is highly expressed in certain brain regions. It is also detected in cultured neuronal, but not in non-neuronal cells. As a first step towards understanding the mechanisms underlying this regulation, genomic clones containing the 5'-flanking region of the human α1-chimaerin transcriptional unit were isolated and characterised. A cluster of multiple transcription start sites of α1-chimaerin mRNAs was detected by primer-extension and S1-mapping analyses. The cluster was mapped to nucleotides -464 to -434 (relative to nucleotide A in the initiation codon) in genomic DNA. The 5'-proximal region contained no TATA box, initiator motif and Sp1-binding site. A 210-bp fragment with approximately 110 bp 5'-flanking sequence could function as a minimal promoter upon analysis using hybrid chloramphenicol acetyltransferase reporter constructs and transient transfection. Internal deletion and point-mutation experiments revealed that a GGCCAATC sequence located at nucleotides -519 to -512 was essential for α1-chimaerin promoter activity. Mobility-shift assay showed the specific binding of nuclear factor(s) to this region, which was competed by the oligonucleotides corresponding to wild-type but not mutant forms. The data also suggest the existence of possible novel CCAAT-binding factor(s) interacting with the α1-chimaerin CCAAT box binding site. A cell-type-preferred suppressor located in the 5'-distal region was found which may play a role in controlling neuron-specific expression of α1-chimaerin mRNA. These findings of a specific promoter for α1-chimaerin transcription will facilitate further studies on its neuronal-specific expression and function.
Source Title: European Journal of Biochemistry
ISSN: 00142956
DOI: 10.1111/j.1432-1033.1995.tb20183.x
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Jan 2, 2018

Page view(s)

checked on Oct 19, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.