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Title: Oct4 switches partnering from Sox2 to Sox17 to reinterpret the enhancer code and specify endoderm
Authors: Aksoy, I.
Jauch, R.
Chen, J.
Dyla, M.
Divakar, U.
Bogu, G.K.
Teo, R.
Leng Ng, C.K.
Herath, W.
Lili, S.
Hutchins, A.P.
Robson, P.
Kolatkar, P.R. 
Stanton, L.W.
Keywords: endoderm
enhancer code
lineage specification
Sox/Oct interaction
Issue Date: 3-Apr-2013
Citation: Aksoy, I., Jauch, R., Chen, J., Dyla, M., Divakar, U., Bogu, G.K., Teo, R., Leng Ng, C.K., Herath, W., Lili, S., Hutchins, A.P., Robson, P., Kolatkar, P.R., Stanton, L.W. (2013-04-03). Oct4 switches partnering from Sox2 to Sox17 to reinterpret the enhancer code and specify endoderm. EMBO Journal 32 (7) : 938-953. ScholarBank@NUS Repository.
Abstract: How regulatory information is encoded in the genome is poorly understood and poses a challenge when studying biological processes. We demonstrate here that genomic redistribution of Oct4 by alternative partnering with Sox2 and Sox17 is a fundamental regulatory event of endodermal specification. We show that Sox17 partners with Oct4 and binds to a unique 'compressed' Sox/Oct motif that earmarks endodermal genes. This is in contrast to the pluripotent state where Oct4 selectively partners with Sox2 at 'canonical' binding sites. The distinct selection of binding sites by alternative Sox/Oct partnering is underscored by our demonstration that rationally point-mutated Sox17 partners with Oct4 on pluripotency genes earmarked by the canonical Sox/Oct motif. In an endodermal differentiation assay, we demonstrate that the compressed motif is required for proper expression of endodermal genes. Evidently, Oct4 drives alternative developmental programs by switching Sox partners that affects enhancer selection, leading to either an endodermal or pluripotent cell fate. This work provides insights in understanding cell fate transcriptional regulation by highlighting the direct link between the DNA sequence of an enhancer and a developmental outcome. © 2013 European Molecular Biology Organization.
Source Title: EMBO Journal
ISSN: 02614189
DOI: 10.1038/emboj.2013.31
Appears in Collections:Staff Publications

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