Please use this identifier to cite or link to this item: https://doi.org/10.1007/s10529-014-1486-5
Title: Improved dominant selection markers and co-culturing conditions for efficient Agrobacterium tumefaciens-mediated transformation of Ustilago scitaminea
Authors: Sun, L.
Yan, M.
Ding, Z.
Liu, Y.
Du, M.
Xi, P.
Liao, J.
Ji, L. 
Jiang, Z.
Keywords: Agrobacterium tumefaciens-mediated transformation
Codon-optimized hygromycin B phosphotransferase gene
Recalcitrant transformation
Sugar-cane smut disease
Ustilago scitaminea
Issue Date: 2014
Citation: Sun, L., Yan, M., Ding, Z., Liu, Y., Du, M., Xi, P., Liao, J., Ji, L., Jiang, Z. (2014). Improved dominant selection markers and co-culturing conditions for efficient Agrobacterium tumefaciens-mediated transformation of Ustilago scitaminea. Biotechnology Letters 36 (6) : 1309-1314. ScholarBank@NUS Repository. https://doi.org/10.1007/s10529-014-1486-5
Abstract: Ustilago scitaminea is the causal agent of sugar-cane smut disease. There is, however, no genetic transformation method for it. Here we report the development of an efficient mutagenesis method based on Agrobacterium tumefaciens-mediated transformation. To improve transformation efficiency, a range of conditions, including the codon-usage preference of the selection marker gene, promoters and the culture conditions for transformation were optimized. A strong promoter to drive marker gene expression, optimized codon usage of selection marker gene, controlled water content and pH of co-culture medium were critical factors affecting transformation efficiency. Our findings provide a useful tool for genetic analysis of this important plant pathogen. © 2014 Springer Science+Business Media Dordrecht.
Source Title: Biotechnology Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/116401
ISSN: 15736776
DOI: 10.1007/s10529-014-1486-5
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