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|Title:||Bioconversion of crude glycerol to glycolipids in Ustilago maydis|
|Citation:||Liu, Y., Koh, C.M.J., Ji, L. (2011-02). Bioconversion of crude glycerol to glycolipids in Ustilago maydis. Bioresource Technology 102 (4) : 3927-3933. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biortech.2010.11.115|
|Abstract:||Ustilago maydis is known to produce glycolipid-type biosurfactants. Here, we show that U. maydis is able to efficiently convert biodiesel-derived crude glycerol to glycolipids. We have optimized the medium composition and environmental factors for bioconversion of crude glycerol to glycolipids. The synthetic medium (MinCG) contains 50gL-1 crude glycerol and 20.3mgL-1 ammonium citrate as the carbon and nitrogen sources, respectively. The supplementation of trace amount of amino acids, Group-B vitamins and precursors of glycolipids, mannose and erythritol, also improved the final yield. At pH 4.0 and 30°C, 32.1gL-1 total glycolipids was produced in a 8.2-day fed-batch bioprocess. Methanol at 2% or above severely inhibited cell growth and production of glycolipids. Our results suggest that U. maydis is an excellent host for the bioconversion of crude glycerol to value-added products. © 2010 Elsevier Ltd.|
|Source Title:||Bioresource Technology|
|Appears in Collections:||Staff Publications|
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