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|Title:||A fast assay for developing serum free media for cord blood haematopoietic cells|
|Authors:||Oh, S.K.W. |
|Citation:||Oh, S.K.W., Ng, T., See, L.H. (1995-08). A fast assay for developing serum free media for cord blood haematopoietic cells. Biotechnology Techniques 9 (8) : 577-582. ScholarBank@NUS Repository. https://doi.org/10.1007/BF00152447|
|Abstract:||One of the bottlenecks in haematopoietic cell expansion is the lack of a defined serum free media. We describe a fast method of monitoring live haematopoietic cell numbers, using fluorescein diacetate, to facilitate the development of such a media. Several carbohydrates (fructose at 500μg/ml and pyruvate at 300μg/ml) and lipids (cholesterol and lecithin, both at 10μg/ml) were tested and could replace serum in cord blood haematopoietic cell cultures. © 1995 Chapman & Hall.|
|Source Title:||Biotechnology Techniques|
|Appears in Collections:||Staff Publications|
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