Please use this identifier to cite or link to this item: https://doi.org/10.1007/BF00152447
Title: A fast assay for developing serum free media for cord blood haematopoietic cells
Authors: Oh, S.K.W. 
Ng, T.
See, L.H.
Issue Date: Aug-1995
Citation: Oh, S.K.W., Ng, T., See, L.H. (1995-08). A fast assay for developing serum free media for cord blood haematopoietic cells. Biotechnology Techniques 9 (8) : 577-582. ScholarBank@NUS Repository. https://doi.org/10.1007/BF00152447
Abstract: One of the bottlenecks in haematopoietic cell expansion is the lack of a defined serum free media. We describe a fast method of monitoring live haematopoietic cell numbers, using fluorescein diacetate, to facilitate the development of such a media. Several carbohydrates (fructose at 500μg/ml and pyruvate at 300μg/ml) and lipids (cholesterol and lecithin, both at 10μg/ml) were tested and could replace serum in cord blood haematopoietic cell cultures. © 1995 Chapman & Hall.
Source Title: Biotechnology Techniques
URI: http://scholarbank.nus.edu.sg/handle/10635/116191
ISSN: 0951208X
DOI: 10.1007/BF00152447
Appears in Collections:Staff Publications

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