Please use this identifier to cite or link to this item:
|Title:||RGS16 attenuates Gα(q)-dependent p38 mitogen-activated protein kinase activation by platelet-activating factor|
|Citation:||Zhang, Y., Neo, S.Y., Han, J., Yaw, L.P., Lin, S.-C. (1999-01-29). RGS16 attenuates Gα(q)-dependent p38 mitogen-activated protein kinase activation by platelet-activating factor. Journal of Biological Chemistry 274 (5) : 2851-2857. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.274.5.2851|
|Abstract:||The large gene family encoding the regulators of G protein signaling (RGS) proteins has been implicated in the fine tuning of a variety of cellular events in response to G protein-coupled receptor activation. Several studies have shown that the RGS proteins can attenuate G protein-activated extracellular signal-regulated kinase (ERK) group of mitogen-activated protein kinases. We demonstrate herein that the production of inositol trisphosphate and the activation of the p38 group of mitogen-activated protein kinases by the G protein-coupled platelet-activating factor (PAF) receptor was attenuated by RGS16 in both CHO cells transiently and stably expressing RGS16. The inhibition was not observed with RGS2, RGS5, and a functionally defective form of RGS16, RGS16(R169S/F170C). The PAF-induced p38 and ERK pathways appeared to be preferentially regulated by RGS16 and RGS1, respectively. Overexpression of a constitutively active form of Gα11 (Gα11 Q209L) prevented the RGS16-mediated attenuation of p38 activity, suggesting that Gα(q/11) is involved in PAF activation of p38. The Gα(q/11) involvement is further supported by the observation that p38 activation by PAF was pertussis toxin-insensitive. These results demonstrate for the first time that apart from ERK, p38 activation by a G protein-coupled receptor can be attenuated by an RGS protein and provide further evidence for the specificity of RGS function in G protein signaling pathways.|
|Source Title:||Journal of Biological Chemistry|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Nov 14, 2018
WEB OF SCIENCETM
checked on Nov 6, 2018
checked on Jul 13, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.