Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/115915
Title: RGF-PCR: A technique to isolate different copies of a multi-copy gene
Authors: Kumaresan, G.
Mathavan, S. 
Issue Date: 25-Feb-2002
Source: Kumaresan, G.,Mathavan, S. (2002-02-25). RGF-PCR: A technique to isolate different copies of a multi-copy gene. Current Science 82 (4) : 442-447. ScholarBank@NUS Repository.
Abstract: Isolation of different copies of a multi-copy gene from the genome is a difficult process. To overcome the difficulty, a novel method known as 'Restricted Genomic Fraction-Polymerase Chain Reaction' (RGF-PCR) has been developed. RGF-PCR is a combination of fractionation of the restricted genome and PCR methods. The transposon Mariner-Like Elements (MLEs) occur as multi-copy genes in the silkworm, Bombyx mori genome. Using RGF-PCR method, isolation of different copies of the multi-copy MLE from different loci of the silkworm genome has been demonstrated. Polymorphic copies of MLEs have been isolated from restricted genomic fractions of silkworm, and the nature of polymorphism has been analysed and presented in this paper.
Source Title: Current Science
URI: http://scholarbank.nus.edu.sg/handle/10635/115915
ISSN: 00113891
Appears in Collections:Staff Publications

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