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Title: Cytoplasmic translocation of the retinoblastoma protein disrupts sarcomeric organization
Authors: Araki, K. 
Kawauchi, K.
Hirata, H.
Yamamoto, M.
Taya, Y.
Issue Date: 3-Dec-2013
Source: Araki, K., Kawauchi, K., Hirata, H., Yamamoto, M., Taya, Y. (2013-12-03). Cytoplasmic translocation of the retinoblastoma protein disrupts sarcomeric organization. eLife 2013 (2) : -. ScholarBank@NUS Repository.
Abstract: Skeletal muscle degeneration is a complication arising from a variety of chronic diseases including advanced cancer. Pro-inflammatory cytokine TNF-α plays a pivotal role in mediating cancer-related skeletal muscle degeneration. Here, we show a novel function for retinoblastoma protein (Rb), where Rb causes sarcomeric disorganization. In human skeletal muscle myotubes (HSMMs), up-regulation of cyclin-dependent kinase 4 (CDK4) and concomitant phosphorylation of Rb was induced by TNF-α treatment, resulting in the translocation of phosphorylated Rb to the cytoplasm. Moreover, induced expression of the nuclear exporting signal (NES)-fused form of Rb caused disruption of sarcomeric organization. We identified mammalian diaphanous-related formin 1 (mDia1), a potent actin nucleation factor, as a binding partner of cytoplasmic Rb and found that mDia1 helps maintain the structural integrity of the sarcomere. These results reveal a novel non-nuclear function for Rb and suggest a potential mechanism of TNF-α-induced disruption of sarcomeric organization. © Araki et al.
Source Title: eLife
ISSN: 2050084X
DOI: 10.7554/eLife.01228
Appears in Collections:Staff Publications

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