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|Title:||An integrated approach in the discovery and characterization of a novel nuclear protein over-expressed in liver and pancreatic tumors|
|Authors:||Choong, M.L. |
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|Citation:||Choong, M.L., Tan, L.K., Lo, S.L., Ren, E.-C., Ou, K., Ong, S.-E., Liang, R.C.M.Y., Seow, T.K., Chung, M.C.M. (2001-05-11). An integrated approach in the discovery and characterization of a novel nuclear protein over-expressed in liver and pancreatic tumors. FEBS Letters 496 (2-3) : 109-116. ScholarBank@NUS Repository. https://doi.org/10.1016/S0014-5793(01)02409-7|
|Abstract:||An integrated approach in protein discovery through the use of multidisciplinary tools was reported. A novel protein, Hcc-1, was identified by analysis of the hepatocellular carcinoma (HCC)-M cell proteome. The assembled EST sequence of the 210 amino acid novel protein was subsequently confirmed by rapid amplification of cDNA ends (RACE). A total of 687 bp at the 5′ untranslated region of Hcc-1 was identified. Promoter activity and several upstream open reading frames (uORFs) were demonstrated at this region. Bioinformatics prediction showed that the first 42 amino acids of the protein is a SAP domain with sequence matches to hnRNP from various vertebrate species. The Hcc-1 protein was localized to the cell nucleus while the gene was localized to chromosome 7q22.1. Hcc-1 cDNA level was increased in pancreatic adenocarcinoma. The level was also increased in well-differentiated hepatocellular carcinoma but decreases as the carcinoma progressed to a poorly differentiated stage. © 2001 Published by Elsevier Science B.V.|
|Source Title:||FEBS Letters|
|Appears in Collections:||Staff Publications|
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