Please use this identifier to cite or link to this item:
|Title:||Preparation of culture media for fertilized one-cell mouse eggs.|
|Source:||Carter, D.A. (1993). Preparation of culture media for fertilized one-cell mouse eggs.. Methods in molecular biology (Clifton, N.J.) 18 : 141-143. ScholarBank@NUS Repository.|
|Abstract:||Two types of culture media are required for the in vitro manipulations involved in the process of making transgenic mice. (1) M16: This is used for maintenance of eggs in microdrop cultures in a 37°C incubator gassed with 5% CO. Since this medium is buffered with bicarbonate alone and the eggs are extremely susceptible to pH changes, it is not suitable for maintaining eggs outside the incubator. (2) M2: This is used for maintaining eggs outside the incubator during prolonged manipulations, such as collection or microinjection. M2 has essentially the same components as M16, although the bicarbonate is partially replaced with HEPES buffer to facilitate survival outside the CO incubator. However, the eggs should not be maintained in M2 for periods longer than 30 mins.|
|Source Title:||Methods in molecular biology (Clifton, N.J.)|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jan 19, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.