Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/114791
Title: METABOLIC ENGINEERING OF THE DEOXYXYLULOSE PHOSPHATE PATHWAY FOR ISOPRENOID PRODUCTION IN E.COLI
Authors: ZHANG CONGQIANG
Keywords: Isoprenoids, systematical pathway optimisation, efflux pumps, PTS
Issue Date: 31-Mar-2014
Source: ZHANG CONGQIANG (2014-03-31). METABOLIC ENGINEERING OF THE DEOXYXYLULOSE PHOSPHATE PATHWAY FOR ISOPRENOID PRODUCTION IN E.COLI. ScholarBank@NUS Repository.
Abstract: Metabolic engineering offers a sustainable approach for the production of various compounds from inexpensive and renewable starting materials, including bulk chemicals, fine chemicals, drugs and biofuels. This thesis is focused on developing engineering tools on optimizing strains and global environments to maximize isoprenoid production and attempting to elucidate the underlying biochemical mechanisms. First, precursor was identified as a limiting factor and its availability was increased through engineering carbon uptake pathway. Second, an experimental design aided systematic pathway optimization method was developed to systematically optimize the expression of carbon uptake and isoprenoid pathway genes. Built on the genetically optimized strain, isoprenoid production was further improved by optimizing the medium and cultural conditions, resulting in significant improvement. Third, the efficient transporter screening and engineering method was established, resulting in the significant enhancement of cytotoxic isoprenoid production. Lastly, a semi-closed dual-flux platform was established to monitor the concentration of intracellular and extracellular metabolite intermediates. This platform and the observed correlation of the efflux and intracellular concentration pave the road to understand quantitatively the isoprenoid pathway in the future.
URI: http://scholarbank.nus.edu.sg/handle/10635/114791
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Zhang Congqiang Thesis (Final version) 3.pdf4.78 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

65
checked on Feb 17, 2018

Download(s)

77
checked on Feb 17, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.