Please use this identifier to cite or link to this item:
Title: Transcript scanning reveals novel and extensive splice variations in human L-type voltage-gated calcium channel, Cav1.2 α1 subunit
Authors: Tang, Z.Z.
Liang, M.C.
Lu, S.
Yu, D.
Yu, C.Y.
Yue, D.T.
Soong, T.W. 
Issue Date: 22-Oct-2004
Source: Tang, Z.Z., Liang, M.C., Lu, S., Yu, D., Yu, C.Y., Yue, D.T., Soong, T.W. (2004-10-22). Transcript scanning reveals novel and extensive splice variations in human L-type voltage-gated calcium channel, Cav1.2 α1 subunit. Journal of Biological Chemistry 279 (43) : 44335-44343. ScholarBank@NUS Repository.
Abstract: The L-type (Cav1.2) voltage-gated calcium channels play critical roles in membrane excitability, gene expression, and muscle contraction. The generation of splice variants by the alternative splicing of the pore-forming Cav1.2 α1-subunit (α11.2) may thereby provide potent means to enrich functional diversity. To date, however, no comprehensive scan of α11.2 splice variation has been performed, particularly in the human context. Here we have undertaken such a screen, exploiting recently developed "transcript scanning" methods to probe the human gene. The degree of variation turns out to be surprisingly large; 19 of the 55 exons comprising the human α11.2 gene were subjected to alternative splicing. Two of these are previously unrecognized exons and two others were not known to be spliced. Comparisons of fetal and adult heart and brain uncovered a large IVS3-S4 variability resulting from combinatorial utilization of exons 31-33. Electrophysiological characterization of such IVS3-S4 variation revealed unmistakable shifts in the voltage dependence of activation, according to an interesting correlation between increased IVS3-S4 linker length and activation at more depolarized potentials. Steady-state inactivation profiles remained unaltered. This systematic portrait of splice variation furnishes a reference library for comprehending combinatorial arrangements of Cav1.2 splice exons, especially as they impact development, physiology, and disease.
Source Title: Journal of Biological Chemistry
ISSN: 00219258
DOI: 10.1074/jbc.M407023200
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Feb 28, 2018


checked on Feb 19, 2018

Page view(s)

checked on Mar 12, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.