Please use this identifier to cite or link to this item: https://doi.org/10.1002/(SICI)1097-0215(19990531)81:53.0.CO;2-F
Title: Sodium selenite-induced oxidative stress and apoptosis in human hepatoma HepG2 cells
Authors: Shen, H.-M. 
Yang, C.-F.
Ong, C.-N. 
Issue Date: 1999
Citation: Shen, H.-M.,Yang, C.-F.,Ong, C.-N. (1999). Sodium selenite-induced oxidative stress and apoptosis in human hepatoma HepG2 cells. International Journal of Cancer 81 (5) : 820-828. ScholarBank@NUS Repository. https://doi.org/10.1002/(SICI)1097-0215(19990531)81:53.0.CO;2-F
Abstract: The mechanisms involved in the anti-carcinogenic activity of selenium remain to be elucidated. In the present study, we examined sodium selenite- induced oxidative stress and apoptosis in a human hepatoma cell line (HepG2). Sodium selenite (10 μM) exerted clear cytotoxic effect, as shown by the significant increase of lactate dehydrogenase leakage. Selenite- induced DNA alterations in apoptosis were studied by: 1. comet assay; 2. TdT- mediated dUTP nick end-labeling assay. In addition, characteristic apoptotic morphological alterations were also observed in selenite-treated cells. Our results clearly show that Se-induced cell death occurs predominantly in the form of apoptosis. Selenite-induced oxidative stress was evaluated by the measurement of reactive oxygen species production using lucigenin-dependent chemiluminescence. The involvement of glutathione in selenite-induced oxidative stress was further demonstrated by the concurrent decline of intracellular reduced glutathione and increase of oxidized glutathione contents in Se-treated cells. Moreover, the finding that selenite-induced oxidative stress and apoptosis was significantly attenuated by superoxide dismutase, catalase and deferoxamine provides additional evidence to suggest that Se-induced oxidative stress mediates the induction of apoptosis, a mechanism related to the anti-carcinogenic and chemopreventive effect of Se.
Source Title: International Journal of Cancer
URI: http://scholarbank.nus.edu.sg/handle/10635/113656
ISSN: 00207136
DOI: 10.1002/(SICI)1097-0215(19990531)81:53.0.CO;2-F
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

34
checked on Sep 28, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.