Please use this identifier to cite or link to this item: https://doi.org/10.1074/jbc.M405252200
Title: Expression of the human myotonic dystrophy kinase-related Cdc42-binding kinase γ is regulated by promoter DNA methylation and Sp1 binding
Authors: Ng, Y.
Tan, I.
Lim, L.
Leung, T. 
Issue Date: 13-Aug-2004
Citation: Ng, Y., Tan, I., Lim, L., Leung, T. (2004-08-13). Expression of the human myotonic dystrophy kinase-related Cdc42-binding kinase γ is regulated by promoter DNA methylation and Sp1 binding. Journal of Biological Chemistry 279 (33) : 34156-34164. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.M405252200
Abstract: Myotonic dystrophy kinase-related Cdc42 binding kinasas (MRCKs) are family members most related to the myotonic dystrophy kinase (DMPK), RhoA-binding kinase (ROK), and citron kinase. Two highly conserved members, MRCKα and -β, have been previously identified and characterized. We now describe a novel isoform, MRCKγ, which is functionally and structurally related to members of this kinase family. We show these kinases to have marked similarities in their genomic organization, substrate phosphorylation, and catalytic autoinhibition. Unlike MRCKα and -β, which are expressed ubiquitously, MRCKγ mRNA was only expressed in heart and skeletal muscle. In cultured cells, MRCKγ showed differential expression with high levels of expression only in certain cell lines. DNA analysis showed that lack of expression is correlated with promoter DNA meihylation. We have mapped the methylation sites in the MRCKγ promoter. Significantly, agents that suppressed DNA methylation caused increases in the expression of the kinase in low-expressing cells, further supporting the notion that promoter DNA methylation plays an important role in the expression of MRCKγ. Analysis of the MRCKγ promoter has also revealed two proximal Sp1 sites that are essential for transcriptional activity. We conclude that both promoter DNA methylation and Sp1 binding are important regulators for MRCKγ expression.
Source Title: Journal of Biological Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/113474
ISSN: 00219258
DOI: 10.1074/jbc.M405252200
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

13
checked on Jun 19, 2018

WEB OF SCIENCETM
Citations

10
checked on Jun 11, 2018

Page view(s)

34
checked on Jun 14, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.