Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.cccn.2003.08.012
DC Field | Value | |
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dc.title | Effects of storage time on stability of salivary immunoglobulin A and lysozyme | |
dc.contributor.author | Ng, V. | |
dc.contributor.author | Koh, D. | |
dc.contributor.author | Fu, Q. | |
dc.contributor.author | Chia, S.-E. | |
dc.date.accessioned | 2014-12-01T06:54:43Z | |
dc.date.available | 2014-12-01T06:54:43Z | |
dc.date.issued | 2003-12 | |
dc.identifier.citation | Ng, V., Koh, D., Fu, Q., Chia, S.-E. (2003-12). Effects of storage time on stability of salivary immunoglobulin A and lysozyme. Clinica Chimica Acta 338 (1-2) : 131-134. ScholarBank@NUS Repository. https://doi.org/10.1016/j.cccn.2003.08.012 | |
dc.identifier.issn | 00098981 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/113456 | |
dc.description.abstract | Background: In many research settings, storage of samples prior to analysis is unavoidable. This study investigates the effects of storage time on stability of salivary immunoglobulin A (IgA) and lysozyme. Methods: Saliva samples were obtained from 30 healthy adults. Each sample was divided into five aliquots and stored at -30°C until analysis. The samples were measured for IgA and lysozyme concentrations after 1, 2, 3, 8 and 12 months of storage using enzyme-linked immunosorbent assay. Results: There was a decline in the concentrations of IgA and lysozyme with increasing storage time. Repeated measures analyses for both salivary IgA and lysozyme showed a significant difference after 8 months of storage as compared to the 1st month (p10% after storage for 8 months or more. A similar pattern was observed for lysozyme with % change in majority of the samples >14% levels when the samples were assayed at 8th month and beyond (mean% change±S.D.>14%). Conclusion: Salivary IgA and lysozyme concentrations remain stable for up to 3 months when stored at -30°C. These findings have important implications with regard to measurement validity of salivary biomarkers research. © 2003 Elsevier B.V. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.cccn.2003.08.012 | |
dc.source | Scopus | |
dc.subject | Salivary biomarkers | |
dc.subject | Salivary IgA | |
dc.subject | Salivary lysozyme | |
dc.subject | Stability | |
dc.subject | Storage duration | |
dc.type | Article | |
dc.contributor.department | COMMUNITY,OCCUPATIONAL & FAMILY MEDICINE | |
dc.description.doi | 10.1016/j.cccn.2003.08.012 | |
dc.description.sourcetitle | Clinica Chimica Acta | |
dc.description.volume | 338 | |
dc.description.issue | 1-2 | |
dc.description.page | 131-134 | |
dc.description.coden | CCATA | |
dc.identifier.isiut | 000187025800019 | |
Appears in Collections: | Staff Publications |
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