Please use this identifier to cite or link to this item:
|Title:||Effects of storage time on stability of salivary immunoglobulin A and lysozyme|
|Citation:||Ng, V., Koh, D., Fu, Q., Chia, S.-E. (2003-12). Effects of storage time on stability of salivary immunoglobulin A and lysozyme. Clinica Chimica Acta 338 (1-2) : 131-134. ScholarBank@NUS Repository. https://doi.org/10.1016/j.cccn.2003.08.012|
|Abstract:||Background: In many research settings, storage of samples prior to analysis is unavoidable. This study investigates the effects of storage time on stability of salivary immunoglobulin A (IgA) and lysozyme. Methods: Saliva samples were obtained from 30 healthy adults. Each sample was divided into five aliquots and stored at -30°C until analysis. The samples were measured for IgA and lysozyme concentrations after 1, 2, 3, 8 and 12 months of storage using enzyme-linked immunosorbent assay. Results: There was a decline in the concentrations of IgA and lysozyme with increasing storage time. Repeated measures analyses for both salivary IgA and lysozyme showed a significant difference after 8 months of storage as compared to the 1st month (p10% after storage for 8 months or more. A similar pattern was observed for lysozyme with % change in majority of the samples >14% levels when the samples were assayed at 8th month and beyond (mean% change±S.D.>14%). Conclusion: Salivary IgA and lysozyme concentrations remain stable for up to 3 months when stored at -30°C. These findings have important implications with regard to measurement validity of salivary biomarkers research. © 2003 Elsevier B.V. All rights reserved.|
|Source Title:||Clinica Chimica Acta|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 18, 2018
WEB OF SCIENCETM
checked on Sep 3, 2018
checked on Jun 29, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.