Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.cccn.2004.01.013
Title: Effect of storage conditions on the extraction of PCR-quality genomic DNA from saliva
Authors: Ng, D.P.K. 
Koh, D. 
Choo, S.G.L.
Ng, V.
Fu, Q.
Keywords: Analysis of variance
ANOVA
Deoxyribonucleic acid
DNA
Genomic DNA
Genotyping
GFPT1
Glutamine-fructose-6- phosphate transaminase 1
OD
Optical density
PBS
PCR
Phosphate-buffered saline
Polymerase chain reaction
Saliva
Storage conditions
Issue Date: May-2004
Citation: Ng, D.P.K., Koh, D., Choo, S.G.L., Ng, V., Fu, Q. (2004-05). Effect of storage conditions on the extraction of PCR-quality genomic DNA from saliva. Clinica Chimica Acta 343 (1-2) : 191-194. ScholarBank@NUS Repository. https://doi.org/10.1016/j.cccn.2004.01.013
Abstract: Background: Saliva is a potentially useful source of genomic DNA for genetic studies since it can be collected in a painless and non-invasive manner. We sought to determine whether different storage conditions of saliva samples impact our ability to extract genomic DNA that is of sufficient quality for use in the polymerase chain reaction (PCR). Methods: Saliva was collected from healthy volunteers and 2-ml aliquots subjected to different storage conditions: S1 - washing of saliva using phosphate-buffered saline (PBS) and extraction of DNA on the same day of collection; S2 - washing and centrifugation to yield a pellet, which was stored at-70°C for 1 week prior to DNA extraction; S3 - storage of whole saliva at 4°C for 7 days, followed by washing and extraction of DNA; S4 - storage at 4°C for 7 days, followed by washing and pellet formation. The pellet was stored at -70°C for 1 month before extraction of the DNA; S5 - storage at-70°C for 1 month, followed by washing and extraction of DNA. DNA yield and purity was determined by spectrophotometry at 260 and 280 nm. Twenty nanograms of genomic DNA was used for the polymerase chain reaction, and the resulting PCR band was captured by digital photography and quantified. Results: The amounts of DNA extracted from 2 ml of saliva varied widely under the different storage conditions, while purity of the DNA extraction, based on OD260/280 ratios, was good and comparable. PCR resulted in the presence of a single specific product of the correct size from all samples regardless of saliva storage conditions. Quantification of PCR bands showed significant differences between the various storage conditions (P
Source Title: Clinica Chimica Acta
URI: http://scholarbank.nus.edu.sg/handle/10635/113453
ISSN: 00098981
DOI: 10.1016/j.cccn.2004.01.013
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

46
checked on Dec 11, 2018

WEB OF SCIENCETM
Citations

36
checked on Dec 11, 2018

Page view(s)

47
checked on Dec 7, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.