Please use this identifier to cite or link to this item:
Title: Developmental activation of calmodulin-dependent facilitation of cerebellar P-type Ca2+ current
Authors: Chaudhuri, D.
Alseikhan, B.A.
Chang, S.Y.
Soong, T.W. 
Yue, D.T.
Keywords: α1A
Alternative splicing
Cerebellar Purkinje neuron
EF hand
P/Q-type channel
Short-term synaptic plasticity
Issue Date: 7-Sep-2005
Source: Chaudhuri, D., Alseikhan, B.A., Chang, S.Y., Soong, T.W., Yue, D.T. (2005-09-07). Developmental activation of calmodulin-dependent facilitation of cerebellar P-type Ca2+ current. Journal of Neuroscience 25 (36) : 8282-8294. ScholarBank@NUS Repository.
Abstract: P-type (Cav2.1) Ca2+ channels are a central conduit of neuronal Ca2+ entry, so their Ca2+ feedback regulation promises widespread neurobiological impact. Heterologous expression of recombinant Cav2.1 channels demonstrates that the Ca2+ sensor calmodulin can trigger Ca2+-dependent facilitation (CDF) of channel opening. This facilitation occurs when local Ca2+ influx through individual channels selectively activates the C-terminal lobe of calmodulin. In neurons, however, such calmodulin-mediated processes have yet to be detected, and CDF of native P-type current has thus far appeared different, arguably triggered by other Ca2+ sensing molecules. Here, in cerebellar Purkinje somata abundant with prototypic P-type channels, we find that the C-terminal lobe of calmodulin does produce CDF, and such facilitation augments Ca2+ entry during stimulation by repetitive action-potential and complex-spike waveforms. Beyond recapitulating key features of recombinant channels, these neurons exhibit an additional modulatory dimension: developmental upregulation of CDF during postnatal week 2. This phenomenon reflects increasing somatic expression of Cav2.1 splice variants that manifest CDF and progressive dendritic targeting of variants lacking CDF. Calmodulin-triggered facilitation is thus fundamental to native Ca v2.1 and rapidly enhanced during early development. Copyright © 2005 Society for Neuroscience.
Source Title: Journal of Neuroscience
ISSN: 02706474
DOI: 10.1523/JNEUROSCI.2253-05.2005
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Feb 27, 2018


checked on Feb 14, 2018

Page view(s)

checked on Mar 12, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.