Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/113277
Title: IDENTIFICATION OF INTRINSICALLY DISORDERED SEPTAL PORE ASSOCIATED PROTEINS REGULATING DIVERSE ASPECTS OF FUNGAL CELL BIOLOGY
Authors: JULIAN LAI U-MING
Keywords: filamentous fungi, Neurospora crassa, septal pore, intrinsically disordered proteins, multicellular organization
Issue Date: 22-Jul-2014
Citation: JULIAN LAI U-MING (2014-07-22). IDENTIFICATION OF INTRINSICALLY DISORDERED SEPTAL PORE ASSOCIATED PROTEINS REGULATING DIVERSE ASPECTS OF FUNGAL CELL BIOLOGY. ScholarBank@NUS Repository.
Abstract: Multicellular fungi, like plants and animals, possess cell-to-cell channels (septal pores) that allow intercellular communication. Here, using a combination of mass spectrometry of Woronin body associated proteins and machine learning, I have identified a group of proteins termed septal pore associated (SPA) proteins that localize to the septal pore in rings or pore-centred foci. SPA proteins are not homologous at the primary sequence level but share similar physical characteristics and are predicted to be intrinsically disordered. Some SPA proteins aggregate at septal pores and in vitro through a non-amyloidal mechanism distinct from other intrinsically disordered proteins. SPA deletions result in diverse phenotypes ranging from uncontrolled Woronin body plugging, excessive septation and the failure of septal pore maintenance. These results suggest that the septal pore is an important compartment that is a focal point for self-assembly of disordered proteins, which regulate diverse cellular processes.
URI: http://scholarbank.nus.edu.sg/handle/10635/113277
Appears in Collections:Ph.D Theses (Open)

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