Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0049885
Title: Physical Organization of DNA by Multiple Non-Specific DNA-Binding Modes of Integration Host Factor (IHF)
Authors: Lin, J.
Chen, H. 
Dröge, P.
Yan, J. 
Issue Date: 14-Nov-2012
Citation: Lin, J., Chen, H., Dröge, P., Yan, J. (2012-11-14). Physical Organization of DNA by Multiple Non-Specific DNA-Binding Modes of Integration Host Factor (IHF). PLoS ONE 7 (11) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0049885
Abstract: The integration host factor (IHF) is an abundant nucleoid-associated protein and an essential co-factor for phage λ site-specific recombination and gene regulation in E. coli. Introduction of a sharp DNA kink at specific cognate sites is critical for these functions. Interestingly, the intracellular concentration of IHF is much higher than the concentration needed for site-specific interactions, suggesting that non-specific binding of IHF to DNA plays a role in the physical organization of bacterial chromatin. However, it is unclear how non-specific DNA association contributes to DNA organization. By using a combination of single DNA manipulation and atomic force microscopy imaging methods, we show here that distinct modes of non-specific DNA binding of IHF result in complex global DNA conformations. Changes in KCl and IHF concentrations, as well as tension applied to DNA, dramatically influence the degree of DNA-bending. In addition, IHF can crosslink DNA into a highly compact DNA meshwork that is observed in the presence of magnesium at low concentration of monovalent ions and high IHF-DNA stoichiometries. Our findings provide important insights into how IHF contributes to bacterial chromatin organization, gene regulation, and biofilm formation. © 2012 Lin et al.
Source Title: PLoS ONE
URI: http://scholarbank.nus.edu.sg/handle/10635/113130
ISSN: 19326203
DOI: 10.1371/journal.pone.0049885
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
2012_Physical_Organization_of_DNA_by-published.PDF832.22 kBAdobe PDF

OPEN

PublishedView/Download

SCOPUSTM   
Citations

25
checked on Oct 12, 2018

WEB OF SCIENCETM
Citations

23
checked on Oct 2, 2018

Page view(s)

45
checked on Oct 12, 2018

Download(s)

2
checked on Oct 12, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.