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|Title:||Improved high-force magnetic tweezers for stretching and refolding of proteins and short DNA|
|Authors:||Chen, H. |
|Source:||Chen, H., Fu, H., Zhu, X., Cong, P., Nakamura, F., Yan, J. (2011-01-19). Improved high-force magnetic tweezers for stretching and refolding of proteins and short DNA. Biophysical Journal 100 (2) : 517-523. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bpj.2010.12.3700|
|Abstract:||Although magnetic tweezers have many unique advantages in terms of specificity, throughput, and force stability, this tool has had limited application on short tethers because accurate measurement of force has been difficult for short tethers under large tension. Here, we report a method that allows us to apply magnetic tweezers to stretch short biomolecules with accurate force calibration over a wide range of up to 100 pN. We demonstrate the use of the method by overstretching of a short DNA and unfolding/refolding a protein of filamin A immunoglobulin domains 1-8. Other potential applications of this method are also discussed. © 2011 by the Biophysical Society.|
|Source Title:||Biophysical Journal|
|Appears in Collections:||Staff Publications|
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