Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bpj.2010.12.3700
Title: Improved high-force magnetic tweezers for stretching and refolding of proteins and short DNA
Authors: Chen, H. 
Fu, H. 
Zhu, X. 
Cong, P.
Nakamura, F.
Yan, J. 
Issue Date: 19-Jan-2011
Source: Chen, H., Fu, H., Zhu, X., Cong, P., Nakamura, F., Yan, J. (2011-01-19). Improved high-force magnetic tweezers for stretching and refolding of proteins and short DNA. Biophysical Journal 100 (2) : 517-523. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bpj.2010.12.3700
Abstract: Although magnetic tweezers have many unique advantages in terms of specificity, throughput, and force stability, this tool has had limited application on short tethers because accurate measurement of force has been difficult for short tethers under large tension. Here, we report a method that allows us to apply magnetic tweezers to stretch short biomolecules with accurate force calibration over a wide range of up to 100 pN. We demonstrate the use of the method by overstretching of a short DNA and unfolding/refolding a protein of filamin A immunoglobulin domains 1-8. Other potential applications of this method are also discussed. © 2011 by the Biophysical Society.
Source Title: Biophysical Journal
URI: http://scholarbank.nus.edu.sg/handle/10635/113128
ISSN: 00063495
DOI: 10.1016/j.bpj.2010.12.3700
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