Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/112939
Title: Sequence analysis and in vitro expression of genes 6 and 11 of an ovine group B rotavirus isolate, KB63: Evidence for a non-defective, C-terminally truncated NSP1 and a phosphorylated NSP5
Authors: Shen, S.
McKee, T.A.
Wang, Z.D.
Desselberger, U.
Liu, D.X. 
Issue Date: 1999
Citation: Shen, S.,McKee, T.A.,Wang, Z.D.,Desselberger, U.,Liu, D.X. (1999). Sequence analysis and in vitro expression of genes 6 and 11 of an ovine group B rotavirus isolate, KB63: Evidence for a non-defective, C-terminally truncated NSP1 and a phosphorylated NSP5. Journal of General Virology 80 (8) : 2077-2085. ScholarBank@NUS Repository.
Abstract: An ovine group B rotavirus (GBR) isolate, KB63, was isolated from faeces of a young goat with diarrhoea in Xinjiang, People's Republic of China. Sequence determination and comparison of genes 6 and 11 with the corresponding sequences of GBR strains ADRV and IDIR showed that they were the cognate genes encoding NSP1 and NSP5, respectively. While the overall identities of nucleotide sequences between these two genes and the corresponding genes of strains ADRV and IDIR were in the range 52.6-57.2%, the identities of deduced amino acid sequences were only 34.9-46.3%. These results demonstrate that the substantial diversity of NSP1 observed among group A rotaviruses (GAR) also exists within GBRs and that a high degree of diversity also exists among NSP5 of GBRs, in contrast to GAR NSP5. The NSP1 gene of KB63 contains three ORFs, whereas the NSP1 genes of other GBR strains contain only two. ORFs 2 and 3 of the KB63 gene may be derived from a single ORF corresponding to ORF2 of other GBR strains by the usage of a stop codon created by an upstream single base deletion and single point mutations. In vitro expression studies showed that ORFs 1 and 2, but not 3, of gene 6 can be translated, suggesting that ORF2 may encode a C-terminally truncated, potentially functional product. It may play a role, together with the product of ORF1, in virus replication, as the virus can be passaged further in kids. Similarly, gene 11 can be translated in vitro. Like its counterpart in GARs, the protein encoded by gene 11 was shown to be phosphorylated in vitro.
Source Title: Journal of General Virology
URI: http://scholarbank.nus.edu.sg/handle/10635/112939
ISSN: 00221317
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

35
checked on Nov 16, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.