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|Title:||Fabrication of 3-Dimensional microenvironment based on chitosan under physiological conditions for HepG2 proliferation|
|Citation:||Zhu, J.H.,Lao, X.J.,Ho, H.T.,Yu, H. (2004). Fabrication of 3-Dimensional microenvironment based on chitosan under physiological conditions for HepG2 proliferation. Transactions - 7th World Biomaterials Congress : 1507-. ScholarBank@NUS Repository.|
|Abstract:||To elucidate the effect of positively charged 3-dimensional (3D) microenvironment on cell behaviors in vitro, a new class of microcapsules was fabricated under physiological conditions by utilizing a polyeletrolyte complexation between two oppositely charged, water-soluble polymers. The formed microcapsules have an inner liquid core of half N-acetylated chitosan, which produce the positively charged microenvironment, and an outer shell of methacrylic acid (MAA) (20.4%)-hydroxyethyl methacrylate (HEMA) (27.4%)-methyl methacrylate (MMA) (52.2%) terpolymer (MAA-HEMA-MMA). It was shown that half N-acetylated chitosan, derived from 15.3% N-acetylated chitosans, had good solubility under physiological conditions and supplied enough protonated amino groups to form microcapsules, overcoming the limitation of other chitosan-based microcapsules, which must be prepared at pH less than 6.5. The preparation parameters, including pH, chitosan molar mass, chitosan concentration and reaction time, greatly influenced the intrinsic coating membrane features such as morphology, thickness and network porosity, and further determined microcapsules' permeability and mechanical stability, both of which were measured by the diffusion of FITC-dextran through the coating membrane and the fraction of disrupted microcapsules under shear force respectively. Low concentration of half N-acetylated chitosan favored the permeability of microcapsules, whereas high concentration was good for the resistance of microcapsules to shear force. When HepG2 cells were encapsulated in the microcapsules, a good proliferation rate was shown.|
|Source Title:||Transactions - 7th World Biomaterials Congress|
|Appears in Collections:||Staff Publications|
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