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Title: Quantitative analysis of zinc in rat hippocampal mossy fibers by nuclear microscopy
Authors: Zhang, B.
Ren, M. 
Sheu, F.-S. 
Watt, F. 
Routtenberg, A.
Keywords: Hippocampal mossy fibers
Nuclear microscopy
Quantitative analysis
Zn concentration
Issue Date: Sep-2012
Citation: Zhang, B., Ren, M., Sheu, F.-S., Watt, F., Routtenberg, A. (2012-09). Quantitative analysis of zinc in rat hippocampal mossy fibers by nuclear microscopy. Neuroscience Research 74 (1) : 17-24. ScholarBank@NUS Repository.
Abstract: Zinc (Zn) is involved in regulating mental and motor functions of the brain. Previous approaches have determined Zn content in the brain using semi-quantitative histological methods. We present here an alternative approach to map and quantify Zn levels in the synapses from mossy fibers to CA3 region of the hippocampus. Based on the use of nuclear microscopy, which is a combination of imaging and analysis techniques encompassing scanning transmission ion microscopy (STIM), Rutherford backscattering spectrometry (RBS), and particle induced X-ray emission (PIXE), it enables quantitative elemental mapping down to the parts per million (μg/g dry weight) levels of zinc in rat hippocampal mossy fibers. Our results indicate a laminar-specific Zn concentration of 240 ± 9 μM in wet weight level (135 ± 5 μg/g dry weight) in the stratum lucidum (SL) compared to 144 ± 6 μM in wet weight level (81 ± 3 μg/g dry weight) in the stratum pyramidale (SP) and 78 ± 10 μM in wet weight level (44 ± 5 μg/g dry weight) in the stratum oriens (SO) of the hippocampus. The mossy fibers terminals in CA3 are mainly located in the SL. Hence the Zn concentration is suggested to be within this axonal presynaptic terminal system. © 2012 Elsevier Ireland Ltd and the Japan Neuroscience Society.
Source Title: Neuroscience Research
ISSN: 01680102
DOI: 10.1016/j.neures.2012.06.004
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